Lactarius deterrimus Gröger + Picea

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– Enlarged view –

Literature

references
  Agerer R (1986) Studies on ectomycorrhizae III. Mycorrhizae formed by four fungi in the genera Lactarius and Russula on spruce. Mycotaxon 27: 1-59.
Agerer R (1987) Lactarius deterrimus. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 3, Einhorn-Verlag, Schwäbisch Gmünd.
Franz F (1994) Ektomykorrhizen der Fichte: Identifizierung, Ultrastruktur und Microelementanalyse (EELS, ESI). Diss Univ Bayreuth.
Franz F, Acker G (1995) Rhizomorphs of Picea abies ectomycorrhizae: Ultrastructural aspects and elemental analysis (EELS and ESI) on hyphal inclusions. Nova Hedwigia 60(1-2): 253-267.
Münzenberger B, Metzler B, Kottke I, Oberwinkler F (1986) Morphologische und anatomische Charakterisierung der Mykorrhiza Lactarius deterrimus - Picea abies in vitro. Z Mykol 52(2): 407-422.

Morphology

Morphology of the mycorrhizal system
ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent
exploration type
  medium distance smooth
Morphology of the unramified ends
shape
  straight
or    bent
or    tortuous
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.1 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.32 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.52 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.65 mm    Maximum value
colour
  orange
very tip colour
  orange
older parts colour
  brown
or    orange
or    green
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle secreted latex {after scratching mantle} presence
  present
mantle secreted latex {after scratching mantle} colour-habit
  orange
mantle carbonizing presence
  absent
mantle surface {in general} habit
  smooth
Morphology of rhizomorphs
cross-section shape
  round or roundish
colour
  orange
or    green
ramification kind-frequency
  infrequently, at restricted points
margin habit
  smooth
dimorphism presence
  absent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
Anatomy of laticifers
presence
  present
location
  middle mantle layer
or    inner mantle layer
or    rhizomorphs
shape
  straight and even
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomical features of the mantle
matrix presence
  present
matrix location
  outer mantle layer {apart from tip}
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
matrix kind
  not gelatinous
hyphal system kind
  undifferentiated
septa clamps presence
  absent
cell pigment location-colour
  plasmatically yellowish
and    vacuolarly yellowish
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3.5 µm    Maximum value
cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  without pattern
septa clamps presence
  absent
cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
cell contents presence-kind
  absent
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
10 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
mantle different layers presence
  not discernable
or    discernable
outer mantle layer organisation
  plectenchymatous
middle mantle layer organisation
  plectenchymatous
inner mantle layer organisation
  plectenchymatous
unlayered mantle hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
unlayered mantle hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
3     Maximum value
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
50 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
120 µm    Maximum value
radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
110 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  protruding towards endodermis
or    one or half a row of cortical cells adjoining endodermis free of Hartig net
anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two
structure {in plan view}
  of palmetti type
Anatomy of the mantle in cross-section
mantle different layers presence
  not discernible
outer mantle layer organisation
  plectenchymatous
middle mantle layer organisation
  plectenchymatous
inner mantle layer organisation
  plectenchymatous
unlayered mantle hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
unlayered mantle hyphae radially diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
laticifers diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in cross-section
presence
  present
shape
  tangentially-oval to tangentially-elliptic
tangentially length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  tangentially-oval to tangentially-elliptic
anatomy mantle cross-section cortical (epidermal) cells tangentially length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section cortical (epidermal) cells radially diameter
10 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value

Anatomy of the Hartig net in cross-section
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
intrahyphal hyphae presence
  present
anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  absent
drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Mean (= average)
anatomy emanating elements emanating hyphae cell wall surface habit
  without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Mean (= average)


Anatomy of rhizomorphs
type
  undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
or    differentiated; some hyphae very thick, which appear randomly distributed, pores of septa somtimes enlarged
(type D)
internal nodia presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent
Anatomy of nuclei
number {per cell}
2     Lower value of unspecified range (could be µ-s.d., but not known)
4     Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of siderophilous granulations
presence
  absent
Ultrastructure
septal pores type
  as dolipores with perforated parenthesome

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

reaction with acid fuchsin presence
  absent
reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  present
reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  present
reaction with Melzer's reagent presence
  absent
reaction with phenole presence
  absent
reaction with phenole-aniline presence
  absent
reaction with sulpho-vanillin presence
  present
reaction with toluidin blue presence
  present

Ecology

{occurence in} humus type
  raw humus
geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  forests, woods

Fungus

family
  Russulaceae
subgenus-section
  Lactarius sect. Dapetes
fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

public notes
  Laticifers without septa; autofluorescence of mantle in section with UV-filter blue and laticifers distinctly blue, with blue-filter greenish and laticifers distinctly green; mantle in sulfo-vanillin in general red-brown and laticifers with dark granules, in KOH ochre-orange, especially laticifers, in brillant-cresyl-blue bluish-green and laticifers conspicuously so, in toluidin-blue violet-blue, in cotton-blue slightly blue, in a-naphthol no reaction, in NH4OH ochre-orange and particularly laticifers, in chlorazol-black slightly bluish green, in erythrosin reddish, in fast-green green; nuclei lying close togehter.

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