"Piceirhiza bicolorata" + Picea

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Literature

references
  Brand F, Gronbach E, Taylor AFS (1992) Piceirhiza bicolorata. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 73, Einhorn-Verlag, Schwäbisch Gmünd.
Gronbach E (1988) Charakterisierung und Identifizierung von Ektomykorrhizen in einem Fichtenbestand mit Untersuchungen zur Merkmalsvariabilität in sauer beregneten Flächen. Bibl Mycol 125: 1-216.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  absent
or    monopodial-pinnate
ramification orders
0     Mean (= average)
abundance
  solitary or in small numbers
main axis diameter
0.4 mm    Mean (= average)
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
Morphology of the unramified ends
shape
  straight
or    bent
or    beaded
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.4 mm    Mean (= average)
colour
  black
or    white
very tip colour
  whitish
older parts colour
  black
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  shiny
mantle surface {in detail} kind
  densely woolly
emanating hyphae presence
  present
emanating hyphae abundance
  abundant
emanating hyphae distribution
  concentrated proximally
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
matrix presence
  present
matrix location
  inner mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
hyphal system kind
  forked
and    with rather short, obtuse, even finger-like branches
septa clamps presence
  absent
cell shape
  cylindric, constricted at septa
cell pigment location-colour
  absent
and    membranaceously brownish
cell diameter
2 µm    Mean (= average)
3 µm    Maximum value
cell length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  plectenchymatous
hyphae arrangement
  plectenchymatous, without pattern
cell pigment location-colour
  colourless
or    membranaceously brownish
cell diameter
2 µm    Mean (= average)
3 µm    Maximum value
cell length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
matrix kind
  gelatinous
septa clamps presence
  absent
cell pigment location-colour
  absent
or    membranaceously brownish
cell diameter
2 µm    Mean (= average)
3 µm    Maximum value
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
20 µm    Mean (= average)
mantle thickness {at ectomycorrhizal tip}
20 µm    Mean (= average)
mantle different layers presence
  discernable
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
outer mantle layer hyphae radially diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
middle mantle layer organisation
  plectenchymatous
middle mantle layer hyphae tangentially length
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
middle mantle layer hyphae radially diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
inner mantle layer hyphae radially diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
2     Mean (= average)
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
35 µm    Minimum value
50 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
mean tangenial length TCt
70.3 µm    Mean (= average)
mean shape-ratio TCq
5.7     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
55 µm    Minimum value
70 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
115 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
12 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
86.1 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
4.7     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  protruding towards endodermis
or    in two rows, not reaching endodermis
Anatomy of the mantle in cross-section

Anatomy of the tannin cells in cross-section
presence
  present
rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
3     Maximum value


Anatomy of the Hartig net in cross-section
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
anatomy mantle cross-section hyphal cells around tannin cells thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
anastomoses type
  open, with a short bridge or bridge almost lacking
anastomoses surface habit
  rough or with crystals
anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  membranaceously brownish
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
80 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall surface structures shape
  hemispherical warts
anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)


Anatomy of rhizomorphs
type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

reaction with acid fuchsin presence
  present
reaction with anilin presence
  absent
reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  present
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with phenole presence
  present
reaction with ruthenium-red presence
  absent
reaction with sulpho-vanillin presence
  present

Ecology

geographic occurrence continent
  Europe

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  forests, woods

Remarks

public notes
  Mycorrhizal ends of two colours, upper one third whitish to hyaline, other parts black-brown; mantle in longitudinal section with hyphae tangentially infrequently up to 20 um long; autofluorescence of mantle in section with blue-filter slightly orange; mantle in sulfo-vanillin and in brillant-cresyl-blue with violet hyphal walls, in cotton-blue greenish-brown, in phenole walls olive-brown, in acid fuchsin walls reddish-brown.

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