"Piceirhiza horti-inflata" + Picea

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– Enlarged view –

Literature

references
  Weiss M, Agerer R (1988) Studien an Ektomykorrhizen XII. Drei nichtidentifizierte Mykorrhizen an Picea abies (L.) Karst. aus einer Baumschule. Eur J For Path 18: 26-43.

Morphology

Morphology of the mycorrhizal system
ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  solitary or in small numbers
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
Morphology of the unramified ends
shape
  straight
or    sinuous
or    tortuous
or    beaded
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brown
older parts colour
  brown
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  smooth
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
hyphal system kind
  undifferentiated
septa clamps presence
  absent
cell shape
  cylindric, constricted at septa
cell pigment location-colour
  absent
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
cell wall thickness
0.2 µm    Mean (= average)
1 µm    Maximum value
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
hyphae arrangement
  plectenchymatous, without pattern
Anatomy of the inner mantle layer
hyphae arrangement
  with some considerably inflated cells
septa clamps presence
  absent
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle different layers presence
  not discernable
outer mantle layer organisation
  plectenchymatous

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
80 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
80 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  protruding towards endodermis
anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
anatomy mantle longitudinal section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type
lobes width
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
Anatomy of the mantle in cross-section

Anatomy of the tannin cells in cross-section
presence
  present
tangentially length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells tangentially length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in cross-section
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
or    cylindrical
anatomy mantle cross-section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
or    two
Anatomy of the emanating elements in general
intrahyphal hyphae presence
  absent
anastomoses type
  open, with a long bridge
or    open, with a short bridge or bridge almost lacking
anastomoses surface habit
  rough or with crystals
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  membranaceously brownish
or    membranaceously yellowish
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell diameter
4 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell length
14 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall thickness
-1 µm    Mean (= average)


Anatomy of the warts occouring on emanating hyphae
presence
  present
length
-1 µm    Mean (= average)
2 µm    Maximum value
Anatomy of rhizomorphs
type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  present
{of ectomycorrhiza former} abundance
  occasionally present
{in foreign ectomycorrhizae} presence
  absent
shape
  globular
Anatomy of nuclei
number {per cell}
2     Minimum value
3     Lower value of unspecified range (could be µ-s.d., but not known)
8     Upper value of unspecified range (could be µ+s.d., but not known)
12     Maximum value
shape
  oval
or    elongated
diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present
mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

reaction with acid fuchsin presence
  present
reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  present
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  present
reaction with formol 40% presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with phenole presence
  absent
reaction with phenole-aniline presence
  absent
reaction with sulpho-vanillin presence
  absent
reaction with toluidin blue presence
  present

Ecology

geographic occurrence continent
  Europe

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  nursery

Remarks

public notes
  Mycorrhizal systems monopodial; mycorrhizal ends of root colour; plan view of outer mantle layers with cell diam. of inflated hyphal parts of 10-20 um; autofluorescence of mantle in section with UV-filter slightly yellowish-green, with blue-filter slightly yellow, with green-filter slightly red; mantle in FeSO4 with slightly grey cytoplasm, in brillant-cresyl-blue cytoplasm violet and cell walls without reaction, in toluidin-blue cytoplasm and cell walls bluish violet, in cotton-blue cytoplasm blue and cell walls without reaction, in acid fuchsin cytoplasm slightly red and cell walls without reaction.

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