"Pseudotsugaerhiza baculifera" + Pseudotsuga

– Item listing –

– Enlarged view –

Literature

• references
  Müller WR, Agerer R (1996) "Pseudotsugaerhiza baculifera" + Pseudotsuga menziesii (Mirb.) Franco. Descr Ectomyc 1: 95-100.
Müller WR, Agerer R (1996) Pseudotsugaerhiza baculifera. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 102, Einhorn-Verlag, Schwäbisch Gmünd.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
14 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  nest-like, hyphal mats
• main axis diameter
0.45 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.51 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  abundant
Morphology of the unramified ends
• shape
  sinuous
or    tortuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
12 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.33 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.42 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  yellow
or    white
• very tip colour
  white
• older parts colour
  black
or    grey
or    white
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  yellowish or greenish
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    not smooth
• mantle surface {in detail} kind
  densely woolly
or    loosely stringy
• emanating hyphae presence
  present
• emanating hyphae abundance
  abundant
• emanating hyphae distribution
  not specifically distributed
Morphology of rhizomorphs
• diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
or    flat
• colour
  concolourous to mantle
or    white
• ramification kind-frequency
  repeatedly into smaller filaments
• connection to mantle kind
  oblique
• origin location
  not specific
or    distal
or    proximal
• margin habit
  smooth
or    hairy
or    densely enveloped by hyphae
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
• crystals presence
  present
• crystals location
  emanating hyphae
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
• matrix presence
  present
• matrix location
  inner mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
• hyphal system kind
  undifferentiated
• septa thickness {relative to cell walls}
  as thick as walls
• septa clamps presence
  absent
• cell shape
  cylindric, not constricted at septa
• cell pigment location-colour
  absent
• cell diameter
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7.5 µm    Maximum value
• cell length
5 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Mean (= average)
1 µm    Maximum value
• crystals length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
or    plectenchymatous, with broad streaks of parallel hyphae
• cell pigment location-colour
  colourless
• cell diameter
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• cell contents presence-kind
  absent
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• matrix kind
  gelatinous
• hyphae arrangement
  without pattern
or    with broad streaks of parallel hyphae
• septa clamps presence
  absent
• cell pigment location-colour
  absent
• cell diameter
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell contents presence-kind
  absent
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae radially diameter
3 µm    Minimum value
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
2     Lower value of unspecified range (could be µ-s.d., but not known)
3     Upper value of unspecified range (could be µ+s.d., but not known)
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
30 µm    Minimum value
55 µm    Lower value of unspecified range (could be µ-s.d., but not known)
130 µm    Upper value of unspecified range (could be µ+s.d., but not known)
180 µm    Maximum value
• radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangenial length TCt
91.5 µm    Mean (= average)
• mean shape-ratio TCq
16.6     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
33 µm    Minimum value
50 µm    Lower value of unspecified range (could be µ-s.d., but not known)
105 µm    Upper value of unspecified range (could be µ+s.d., but not known)
127 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
5 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
32 µm    Upper value of unspecified range (could be µ+s.d., but not known)
41 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
76.9 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
3.6     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• structure {in plan view}
  of palmetti type
• lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 µm    Maximum value
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  plectenchymatous
• inner mantle layer organisation
  plectenchymatous

Anatomy of the tannin cells in cross-section
• presence
  present
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
10 µm    Minimum value
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
55 µm    Maximum value
• radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• mean tangential length TCt
29.4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
34.4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean shape-ratio TCq
6.2     Lower value of unspecified range (could be µ-s.d., but not known)
9.9     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
16 µm    Lower value of unspecified range (could be µ-s.d., but not known)
33 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
8 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
27 µm    Upper value of unspecified range (could be µ+s.d., but not known)
32 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
22.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
23.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.3     Lower value of unspecified range (could be µ-s.d., but not known)
1.9     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around tannin cells number
  one
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
• anastomoses type
  open, with a short bridge or bridge almost lacking
or    closed by a simple septum, with a short bridge or bridge almost lacking (contact-septum)
• anastomoses surface habit
  smooth
• anastomoses cell wall thickness {relative to remaining cell walls}
  thicker than
• anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  not striking
or    irregularly inflated or even beaded
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
or    with ellbow-like protrusions
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4.5 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  with crystals
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)
1 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  as thick as
or    moderately thicker
or    considerably thicker, cap-like
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness

Anatomy of the crystals occouring in emanating hyphae
• presence shape
  needle-like
• orientation
  transversely
• length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value

Anatomy of rhizomorphs
• type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
or    undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
• internal nodia presence
  absent
• gelatinous matrix presence
  absent
• gelatinized hyphae presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
• shape
  round
• diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
1 µm    Mean (= average)
• distance {between each other}
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present
• mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

• reaction with acid fuchsin presence
  present
• reaction with anilin presence
  present
• reaction with brillant-cresyl-blue presence
  absent
or    present
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  present
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with ruthenium-red presence
  absent
• reaction with sulpho-vanillin presence
  absent

Ecology

• geographic occurrence continent
  North America
• knowledge about association with foreign fruitbodies presence
  known

Tree

• plant family
  Pinaceae
• plant genus
  Pseudotsuga
• plant habitat kind
  forests, woods

Remarks

• public notes
  Autofluorescence of mantle in section with UV-filter bluish, with blue-filter slightly orange, with green-filter slighly orange; in sulfo-vanillin crystals dissolving, in KOH densely aggregated hyphae slightly brownish, in brillant-cresyl-blue once and not reproducible reddish, in cotton- blue cytoplasm slightly blue, in anilin walls pink, in acid fuchsin walls red.

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