Tricholoma sulphureum (Bull. : Fr.) Kummer + Picea

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Literature

references
  Agerer R (1987) Studies on ectomycorrhizae IX. Mycorrhizae formed by Tricholoma sulfureum and T. vaccinum on spruce. Mycotaxon 28: 327-360.
Agerer R (1987) Tricholoma sulphureum. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 9, Einhorn-Verlag, Schwäbisch Gmünd.

Morphology

Morphology of the mycorrhizal system
ramification presence-type
  monopodial-pinnate
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  abundant
exploration type
  medium distance fringe
Morphology of the unramified ends
shape
  straight
shape {of distal end}
  not inflated, cylindric
length
2 mm    Mean (= average)
diameter
0.3 mm    Mean (= average)
0.33 mm    Maximum value
colour
  white
very tip colour
  whitish
older parts colour
  dark brown
or    brown
or    ochre, yellowish brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
or    not smooth
mantle surface {in detail} kind
  loosely stringy
emanating hyphae presence
  present
emanating hyphae abundance
  abundant
emanating hyphae distribution
  not specifically distributed
Morphology of rhizomorphs
cross-section shape
  round or roundish
or    flat
ramification kind-frequency
  frequently, at restricted points
connection to mantle kind
  distinct
or    oblique
origin location
  proximal
margin habit
  hairy
dimorphism presence
  absent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  ring-like arrangement of hyphal bundles (type A)
septa clamps presence
  absent
cell shape
  cylindric, not constricted at septa
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  plectenchymatous
Anatomy of the inner mantle layer
organisation
  plectenchymatous
or    plectenchymatous with pseudoparenchymatous nests of cells
hyphae arrangement
  ring-like
septa clamps presence
  absent
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
5 µm    Minimum value
10 µm    Mean (= average)
15 µm    Maximum value
mantle thickness {at ectomycorrhizal tip}
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle different layers presence
  discernable
outer mantle layer hyphae tangentially length
3 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer hyphae tangentially length
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
inner mantle layer hyphae radially diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  present
shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
or    tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
18 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
80 µm    Upper value of unspecified range (could be µ+s.d., but not known)
105 µm    Maximum value
radially diameter
4 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
17 µm    Maximum value
mean tangenial length TCt
56.5 µm    Mean (= average)
mean shape-ratio TCq
6.7     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
20 µm    Minimum value
35 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
102 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
8 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
32 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
62.2 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
3.1     Mean (= average)

Anatomy of the mantle in cross-section
mantle different layers presence
  discernible
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
outer mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
middle mantle layer organisation
  plectenchymatous
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value

Anatomy of the tannin cells in cross-section
presence
  present
rows number
1     Minimum value
2     Mean (= average)
3     Maximum value
shape
  tangentially-oval to tangentially-elliptic
tangentially length
15 µm    Minimum value
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
radially diameter
4 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
17 µm    Maximum value
mean tangential length TCt
35.4 µm    Mean (= average)
mean shape-ratio TCq
3.8     Mean (= average)

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  tangentially-oval to tangentially-elliptic
anatomy mantle cross-section cortical (epidermal) cells tangentially length
15 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells radially diameter
10 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
29.4 µm    Mean (= average)
anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.5     Mean (= average)

Anatomy of the Hartig net in cross-section
presence
  present
kind
  protruding towards endodermis
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
or    cylindrical
anatomy mantle cross-section hyphal cells around tannin cells thickness
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
anatomy mantle cross-section hyphal rows around tannin cells number
  two
or    three
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type
Anatomy of the emanating elements in general
septal pores configuration
  globular thickenings
anastomoses type
  closed by a simple septum, with a short bridge or bridge almost lacking (contact-septum)
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  acute

Anatomy of the septa of emanating hyphae
clamps presence
  present

Anatomy of secondary septa of emanating hyphae
presence
  present
abundance
  abundant
distribution
  evenly distributed

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Mean (= average)
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
internal nodia presence
  absent
gelatinous matrix presence
  absent
gelatinized hyphae presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent
Anatomy of nuclei
number {per cell}
2     Mean (= average)
Anatomy of siderophilous granulations
presence
  present
frequency
  in few cells
arrangement
  very dense

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

reaction with acid fuchsin presence
  absent
reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  present
reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with phenole presence
  absent
reaction with phenole-aniline presence
  absent
reaction with sulpho-vanillin presence
  absent
reaction with toluidin blue presence
  present

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  forests, woods

Fungus

family
  Tricholomataceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

public notes
  Autofluorescence of mantle in section with UV-filter distinctly greenish yellow, with blue-filter distinctly yellow; mantle in brillant-cresyl-blue bright blue and after washing with water partially violet-blue, in toluidin-blue violet-blue, in cotton-blue hyphal walls slightly blue, in NH4OH no reaction, in chlorazol-black greyish blue.

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