Tuber melanosporum Vitt. + Corylus

– Item listing –

– Enlarged view –

Literature

references
  Rauscher T, Agerer R, Chevalier G (1995) Ektomykorrhizen von Tuber melanosporum, Tuber mesentericum und Tuber rufum (Tuberales) an Corylus avellana. Nova Hedwigia 61: 281-322.
Rauscher T, Chevalier G (1995) Tuber melanosporum. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 87, Einhorn-Verlag, Schwäbisch Gmünd.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pyramidal
ramification orders
1     Mean (= average)
2     Maximum value
abundance
  solitary or in small numbers
main axis diameter
0.33 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.35 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
exploration type
  short distance
Morphology of the unramified ends
shape
  straight
or    bent
or    beaded
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.29 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.33 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brown
or    red
very tip colour
  brownish
or    yellowish
older parts colour
  dark brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  shiny
or    smooth
mantle surface {in detail} kind
  loosely woolly
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  not specifically distributed
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  cystidia
emanating elements cystidia location
  on outer mantle layer
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
organisation {if pseudoparenchymatous} cell shape
  epidermoid, puzzle-like, jig-saw-shaped
mantle type
  epidermoid cells bearing a hyphal net (type Q)
hyphal net cells shape
  not specialized
hyphal net cells diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
hyphal net cell walls habit
  distinct, rather thick
pores between cells presence
  present
pores between cells abundance
  infrequent
septa thickness {relative to cell walls}
  as thick as walls
septa clamps presence
  absent
cell pigment location-colour
  absent
and    membranaceously brownish
cell contents presence-kind
  absent
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
cell length
6 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
17 µm    Maximum value
cell density
10     Lower value of unspecified range (could be µ-s.d., but not known)
11     Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall with globular thickenings
  absent
cell wall surface habit
  smooth
cell wall projections presence
  present
cell wall projections abundance
  infrequent
cell wall projections shape
  even in thickness
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  pseudoparenchymatous
cell pigment location-colour
  membranaceously brownish
or    epimembranaceously yellowish
cell diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
cell length
6 µm    Minimum value
9 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
18 µm    Maximum value
cell density
9     Lower value of unspecified range (could be µ-s.d., but not known)
10     Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
cell wall thickness
0.5 µm    Minimum value
1 µm    Mean (= average)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  pseudoparenchymatous
cell pigment location-colour
  membranaceously brownish
cell diameter
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
11 µm    Maximum value
cell length
4 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  pseudoparenchymatous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
12     Lower value of unspecified range (could be µ-s.d., but not known)
13     Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle different layers presence
  discernable
outer mantle layer organisation
  pseudoparenchymatous
outer mantle layer hyphae tangentially length
3 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
outer mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3.5 µm    Maximum value
middle mantle layer organisation
  pseudoparenchymatous
middle mantle layer hyphae tangentially length
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
13 µm    Maximum value
middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  pseudoparenchymatous
inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  absent

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic, oriented obliquely
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
9 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
35 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
54 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
13.2 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.3     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  periepidermal
structure {in plan view}
  of palmetti type
or    infrequently lobed
lobes width
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section

Anatomy of the tannin cells in cross-section
presence
  absent

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    radially-oval to -elliptic
anatomy mantle cross-section cortical (epidermal) cells tangentially length
9 µm    Minimum value
13 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
23 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells radially diameter
11 µm    Minimum value
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
14.6 µm    Mean (= average)
anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
0.7     Mean (= average)

Anatomy of the Hartig net in cross-section
presence
  present
kind
  apparently two rows deep
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Mean (= average)
3.5 µm    Maximum value
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
lobes width
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
structure {in plan view}
  of palmetti type
or    infrequently lobed
Anatomy of the emanating elements in general
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  awl-shaped, bristle-like (type A)
ramification presence-position
  proximal
or    medial
or    distal
branches ramification presence
  absent
or    present
septa presence
  present
septa kind
  simple
diameter {proximal}
3.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter {distal}
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
290 µm    Upper value of unspecified range (could be µ+s.d., but not known)
420 µm    Maximum value
cell wall colour
  yellowish
or    brownish
cell wall thickness
0.3 µm    Minimum value
0.8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness {relative to mantle cells}
  thicker
cell wall evenness
  even in thickness
surface habit
  with ring-like thickenings
contents presence
  absent
contents type
  absent

Anatomy of emanating hyphae






Anatomy of rhizomorphs
type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  present
{of ectomycorrhiza former} abundance
  occasionally present
shape
  globular
or    as small racemes
Anatomy of nuclei
number {per cell}
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
shape
  oval
diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 µm    Maximum value
length
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 µm    Maximum value
distance {between each other}
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present
mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

reaction with cotton-blue-lactic-acid presence
  present
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  present
reaction with formol 40% presence
  present
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
or    present
reaction with Melzer's reagent presence
  absent
amyloidity haustoria presence
  absent
reaction with phenole presence
  absent
reaction with sulpho-vanillin presence
  absent

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Betulaceae
plant genus
  Corylus
plant habitat kind
  nursery

Fungus

family
  Tuberaceae
fruitbodies growth habit
  hypogeous

Remarks

public notes
  Autofluorescence of mantle in section with UV-filter outer portions sligtly brown and remaining parts dark brown, with blue-filter outer portions yellow-brown and remaining parts dark brown, with green-filter outer portions slightly red and remaining parts dark red; mantle in FeSO4 with greyish-brown walls, in lactic acid and formol walls of lighter colour, in cotton-blue only outermost cell walls slightly blue.

– Item listing –