Arcangeliella borziana Cavara + Picea

– Item listing –

Literature

references
  Egli S, Ayer F, Peter M, Schneider B (2001) Arcangeliella borziana Cavara + Picea abies (L.) Karst. Descr Ectomyc 5: 9-14.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate [often irregular and simple]
or    monopodial-pyramidal [often irregular and simple]
tips {per 10 mm} number
  unknown
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  unknown
main axis diameter
0.45 mm    Minimum value
0.6 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
taste {after 2-3 min chewing}
  unknown
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
exploration type
  contact
Morphology of the unramified ends
mantle hydrophobicity presence
  unknown
shape
  straight
or    bent [slightly]
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.45 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.55 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  orange [red orange to dark salmon]
or    red [red orange to dark salmon]
very tip colour
  unknown
after injury colour
  unknown
older parts colour
  brownish
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  unknown
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  unknown
mantle secreted latex {after scratching mantle} colour-habit
  unknown
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  smooth
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  unknown
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  emanating hyphae
or    cystidia
emanating elements cystidia location
  on outer mantle layer
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  present
location
  inner mantle layer
shape
  unknown
ramification presence-abundance
  unknown
septa presence
  unknown
contents colour
  colourless
cell length
  unknown
cell diameter
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
  unknown
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  unknown
Anatomical features of the mantle
completeness
  complete
matrix presence
  unknown
matrix location
  unknown
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
organisation {if pseudoparenchymatous} cell shape
  angular
mantle type
  angular cells bearing a hyphal net (type P)
hyphal net cells shape
  unknown
hyphal net cells diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
hyphal net cell walls habit
  unknown
hyphal net clamps presence
  absent
matrix kind
  unknown
hyphae arrangement
  not specific
hyphae sticky matrix on hyphal surface presence
  unknown
pores between cells presence
  unknown
pores between cells abundance
  unknown
septa thickness {relative to cell walls}
  unknown
septa clamps presence
  absent
cell pigment location-colour
  unknown
cell contents presence-kind
  unknown
cell diameter
6 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
24 µm    Maximum value
cell length
6 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
24 µm    Maximum value
cell density
  unknown
cell wall thickness
0.1 µm    Mean (= average)
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
cell wall with globular thickenings
  absent
cell wall projections presence
  unknown
cell wall projections abundance
  unknown
cell wall projections shape
  unknown
drops of exuded pigment presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  absent
Anatomy of the inner mantle layer
organisation
  plectenchymatous
matrix kind
  unknown
hyphae arrangement
  without pattern
septa thickness
  unknown
septa clamps presence
  absent
cell pigment location-colour
  unknown
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
  unknown
cell contents presence-kind
  unknown
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
35 µm    Minimum value
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
75 µm    Maximum value
mantle thickness {at ectomycorrhizal tip}
35 µm    Minimum value
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
75 µm    Maximum value
calyptra cell remnants presence
  unknown
calyptra cell remnants type
  unknown
mantle different layers presence
  discernable
outer mantle layer organisation
  pseudoparenchymatous
middle mantle layer presence
  not applicable
middle mantle layer organisation
  not applicable
middle mantle layer hyphae tangentially length
  not applicable
middle mantle layer hyphae radially diameter
  not applicable
inner mantle layer organisation
  plectenchymatous
laticifers diameter
  unknown

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
  unknown
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
radially diameter
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
70 µm    Lower value of unspecified range (could be µ-s.d., but not known)
110 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  protruding towards endodermis
anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type
lobes width
  unknown
lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae clamps presence
  absent
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  thin-walled, slightly tapering, often rather similar to ends of normal hyphae, but mostly originating from a pseudoparenchyma (type O)
branches ramification presence
  absent
septa presence
  absent
basal clamp walls abruptly thick
  absent
foot cell presence
  absent
diameter {proximal}
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter {distal}
  unknown
length
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall colour
  absent
cell wall colour {relative to mantle cells}
  unknown
cell wall thickness
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Mean (= average)
cell wall thickness {relative to mantle cells}
  unknown
cell wall evenness
  unknown
surface habit
  smooth
contents presence
  absent

Anatomy of cystidia (type 2)
type
  fusiform (type F) [triangualr to fusiform]
ramification presence-position
  absent
septa presence
  present
septa kind
  simple
septa distance
  unknown
septa number
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
basal clamp walls abruptly thick
  absent
foot cell presence
  absent
diameter {proximal}
  unknown
diameter {distal}
  unknown
length
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall colour
  absent
cell wall colour {relative to mantle cells}
  unknown
cell wall thickness
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Mean (= average)
cell wall evenness
  unknown
cell wall thickness {relative to mantle cells}
  unknown
surface habit
  smooth
contents presence
  absent
Anatomy of emanating hyphae
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  absent

Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell wall thickness
0.1 µm    Mean (= average)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent

Chemical reactions

reaction with brillant-cresyl-blue presence
  absent
reaction with cotton-blue-lactic-acid presence
  absent
reaction with FeSO4 presence
  absent
reaction with formol 40% presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  present [15%: after 10 min a very weak turquoise discoloration at the very tip]
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with sulpho-vanillin presence
  present [carmine to wine-red, turning to dark-red]
reaction with toluidin blue presence
  absent

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  forests, woods

Fungus

family
  Russulaceae
fruitbodies growth habit
  hypogeous
or    gastroid

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
385     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
325     Mean (= average)

Remarks

public notes
  MboI: 260 bp, 200 bp, 125 bp, 100 bp; TaqI: 275 bp, 270 bp, 70 bp, 55 bp

– Item listing –