Russula amoenolens Romagn. + Quercus

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Literature

references
  Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, Universität München. http://edoc.ub.uni-muenchen.de/archive/00003175/01/Beenken_Ludwig.pdf

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  absent
or    monopodial-pinnate [irregularly]
main axis diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent
exploration type
  medium distance smooth
Morphology of the unramified ends
mantle hydrophobicity presence
  present
shape
  straight
or    sinuous [slightly]
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brownish
or    ochre, yellowish brown
very tip colour
  brownish [lighter than remaining parts]
after injury colour
  unchanged
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
or    covered with soil particles
or    not smooth
mantle surface {in detail} kind
  densely short-spiny
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
Morphology of rhizomorphs
diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  cushion-shaped (semicircular)
colour
  brownish
or    ochre, yellowish brown
ramification kind-frequency
  infrequently, at restricted points
connection to mantle kind
  distinct
origin location
  proximal
connection to substrate
  almost consistently growing along roots
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    emanating hyphae
or    cystidia
emanating elements cystidia location
  generally present
or    on outer mantle layer
or    on rhizomorphs
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
completeness
  complete
matrix presence
  present
matrix location
  outer mantle layer {apart from tip}
or    middle mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae arranged net-like, with prominent cystidia (type D)
matrix kind
  gelatinous
septa clamps presence
  absent
cell pigment location-colour
  absent
and    membranaceously yellowish
cell contents presence-kind
  absent
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Mean (= average)
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
roundish plasmatically yellow cells presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  present
organisation
  plectenchymatous [densely]
or    pseudoparenchymatous
matrix kind
  gelatinous
hyphae arrangement
  plectenchymatous, without pattern
cell diameter
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
13 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous [densely]
hyphae arrangement
  without pattern
hyphae vessel-like presence
  absent
septa clamps presence
  absent
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  gelatinous [cells of mittle mantle layer smaller than those of remaining parts, 4-25x2-5 µm; cells of inner mantle layer smaller than those of remaining parts, 4-30x1,5-2,5 µm]
Anatomy of the mantle in longitudinal section



Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae clamps presence
  absent
anastomoses type
  open, with a short bridge or bridge almost lacking
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  flask-shaped with an apical knob (type D)
apical knobs number
1     Mean (= average)
apical knobs arrangement
  solitary, not in chains
or    symmetrically
apical knobs shape
  globose
or    ovoid [subglobose]
apical knobs diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
apical knobs length
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
apical knobs wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
apical knobs cell wall thickness {relative to cystidial body}
  thicker than those of cystidial body
septa presence
  absent [very infrequent]
septa kind
  simple
septa number
1     Mean (= average)
foot cell presence
  absent
diameter {proximal}
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter {distal}
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
26 µm    Upper value of unspecified range (could be µ+s.d., but not known)
35 µm    Maximum value
cell wall colour
  absent
or    yellowish
cell wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
surface habit
  smooth
contents presence
  present
contents type
  brownish, with latex-like droplets

Anatomy of emanating hyphae
drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Mean (= average)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of the warts occouring on emanating hyphae
presence
  absent
Anatomy of rhizomorphs
type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
nodia presence
  absent
internal nodia presence
  absent
gelatinous matrix presence
  present
gelatinized hyphae presence
  absent
hyphae distinctly different diameter
  present
cup-like structures on surface presence
  absent
conical young side-branches presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
contents presence-kind
  absent
ampullate, trumpet-like inflated presence
  absent
roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present [very infrequent]
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
8 µm    Mean (= average)
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present [very infrequent]
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
8 µm    Mean (= average)
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present [very infrequent]
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
8 µm    Mean (= average)
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present [very infrequent]
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
8 µm    Mean (= average)
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present [very infrequent]
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
8 µm    Mean (= average)
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of chlamydospores
presence
  absent

Chemical reactions

reaction with cotton-blue-lactic-acid presence
  present [cell walls blue]
reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with sulpho-vanillin presence
  present [contents of some cystidia darb brown]

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Fagaceae
plant genus
  Quercus
plant habitat kind
  forests, woods

Fungus

family
  Russulaceae
subgenus-section
  Russula sect. Ingratae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme Alu bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
500     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
220     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
3     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
340     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
260     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 3. band from below number
115     Mean (= average)

Remarks

public notes
  ECoR I: 390 bp, 330 bp; Ray I: 290 bp, 280 bp, 95 bp, 60 bp

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