Laccaria amethystina (Bolt.: Hooker) Murr. + Fagus

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Literature

references
  Brand F (1988) Laccaria amethystina. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 18, Einhorn-Verlag, Schwäbisch Gmünd.
Brand F, Agerer R (1986) Studien an Ektomykorrhizen VIII. Die Mykorrhizen von Lactarius subdulcis, Lactarius vellereus und Laccaria amethystina an Buche. Z Mykol 52: 287-320.
Meyer FH (1963) Laccaria amethystina (Bolt. ex Fr.) Berk. et Br., ein zur Mykorrhizabildung an der Buche befähigter Pilz. Ber Dtsch Bot Ges 76: 90-96.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.55 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent
exploration type
  medium distance smooth
Morphology of the unramified ends
shape
  straight
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.35 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brown
or    ochre, yellowish brown
or    grey
very tip colour
  violet, dark reddish blue
older parts colour
  brown
or    ochre, yellowish brown
or    grey
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  not smooth
mantle surface {in detail} kind
  loosely cottony
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  concentrated proximally
Morphology of rhizomorphs
cross-section shape
  round or roundish
colour
  violet, dark reddish blue
or    grey
or    whitish
ramification kind-frequency
  infrequently, at restricted points
origin location
  proximal
margin habit
  hairy
dimorphism presence
  absent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
hyphal system kind
  with rather short, obtuse, even finger-like branches
septa thickness {relative to cell walls}
  as thick as walls
cell shape
  cylindric, not constricted at septa
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.2 µm    Mean (= average)
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  plectenchymatous
hyphae arrangement
  plectenchymatous, without pattern
cell diameter
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
cell wall thickness
0.2 µm    Mean (= average)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  ring-like
septa clamps presence
  absent
cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
70 µm    Maximum value
mantle different layers presence
  discernable
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  absent


Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  periepidermal
or    in two rows, not reaching endodermis
Anatomy of the mantle in cross-section
mantle different layers presence
  discernible
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3.8 µm    Maximum value
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value

Anatomy of the tannin cells in cross-section
presence
  absent


Anatomy of the Hartig net in cross-section
presence
  present
kind
  apparently two rows deep
or    apparently one and a half row deep
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  cylindrical
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
lobes width
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
structure {in plan view}
  of palmetti type
Anatomy of the emanating elements in general
anastomoses type
  open, with a short bridge or bridge almost lacking
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
anastomoses anastomosal bridge thickness {relative to hyphae}
  thinner
or    as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
distance {from septum}
  adjacent to septum
or    one or two hyphal diam. below the septum

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps outline {in lateral view}
  less than a semicircle
or    not constricted at contact point to subtending hyphal cell
clamps width {relative to hypha in lateral view}
  thinner than
or    as broad as
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  absent

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell length
70 µm    Lower value of unspecified range (could be µ-s.d., but not known)
200 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Mean (= average)


Anatomy of rhizomorphs
type
  undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
internal nodia presence
  absent
gelatinized hyphae presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent
Anatomy of nuclei
number {per cell}
2     Mean (= average)
shape
  round
diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  present
reaction with ethanol 70% presence
  present
reaction with FeSO4 presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  present
reaction with Melzer's reagent presence
  absent
reaction with phenole presence
  present
reaction with ruthenium-red presence
  absent
reaction with sudan III presence
  absent
reaction with sulpho-vanillin presence
  absent
reaction with toluidin blue presence
  present

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Fagaceae
plant genus
  Fagus
plant habitat kind
  forests, woods

Fungus

family
  Tricholomataceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

public notes
  Autofluorescence of mantle in section with UV-filter inclusive emanating hyphae light blue, with blue-filter inclusive emanating hyphae greenish orange; mantle in KOH pigment not bleaching rather becoming more intense, in lactic acid violet pigment bleaching and at first becoming vinaceous, in brillant-cresyl-blue walls violet, in toluidin-blue blue-violet, in cotton-blue walls bluish, in ethanol violet pigment bleaching, in phenole violet pigment bleaching, in chlorazol-black blue-grey.

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