Tuber magnatum Pico + Quercus

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Literature

• references
  Zambonelli A, Salomoni S, Pisi A (1993) Caratterizzazione anatomo-morfologica delle micorrize di Tuber spp. su Quercus pubescens Willd. Micol Ital (3): 73- 90.
Rubini A, Paolocci F, Granetti B, Arcioni S (2001) Morphological characterization of molecular-typed Tuber magnatum ectomycorrhizae. Mycorrhiza 11(4): 179-185.

Morphology

Morphology of the mycorrhizal system
• ramification presence-type
  absent
or    monopodial-pinnate
or    monopodial-pyramidal
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
• exploration type
  short distance
Morphology of the unramified ends
• shape
  straight
• shape {of distal end}
  not inflated, cylindric
or    inflated, club-shaped
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
6 mm    Maximum value
• diameter
0.22 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.26 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown
or    ochre, yellowish brown
• very tip colour
  ochre, yellowish brown
or    whitish
• older parts colour
  brown
• mantle {distinct} surface visibility
  present
• mantle laticifers visibility
  absent
• mantle secreted latex {after scratching mantle} presence
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  not smooth
• mantle surface {in detail} kind
  densely short-spiny
• emanating hyphae presence
  present
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  cystidia
• emanating elements cystidia location
  on outer mantle layer
• crystals presence
  present
• crystals location
  emanating hyphae
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
• anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  epidermoid, puzzle-like, jig-saw-shaped
• mantle type
  epidermoid cells (type M)
• septa clamps presence
  absent
• cell density
4     Minimum value
4.6     Lower value of unspecified range (could be µ-s.d., but not known)
8.1     Upper value of unspecified range (could be µ+s.d., but not known)
10     Maximum value
• cell wall with globular thickenings
  absent
• cell wall projections presence
  present
• cell wall projections abundance
  infrequent
• cell wall projections shape
  thicker at distal end
Anatomy of the inner mantle layer
• organisation
  pseudoparenchymatous
• septa clamps presence
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  pseudoparenchymatous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
4     Minimum value
4.8     Lower value of unspecified range (could be µ-s.d., but not known)
7.4     Upper value of unspecified range (could be µ+s.d., but not known)
8     Maximum value
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
32 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  discernable
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
7.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
13.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
4.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  pseudoparenchymatous
• inner mantle layer organisation
  pseudoparenchymatous
• inner mantle layer hyphae tangentially length
5.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
4.7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)



Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
3.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  pseudoparenchymatous
• inner mantle layer organisation
  pseudoparenchymatous
• inner mantle layer hyphae tangentially length
3.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)



Anatomy of the emanating elements in general
• intrahyphal hyphae clamps presence
  absent
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  awl-shaped, bristle-like (type A)
• ramification presence-position
  absent
or    proximal
• ramification type
  monopodial
• septa presence
  present
• septa kind
  simple
• basal clamp walls abruptly thick
  absent
• diameter {proximal}
2 µm    Minimum value
2.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4.2 µm    Maximum value
• length
42 µm    Minimum value
61 µm    Lower value of unspecified range (could be µ-s.d., but not known)
91.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
120 µm    Maximum value
• cell wall colour
  absent
• surface habit
  smooth
or    warty [Rubini et al. (2001) occasionally with crystals or warts]

Anatomy of emanating hyphae
• shape
  wavy
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  approximately 90°
or    Y-shaped
• distance {from septum}
  adjacent to septum
• side-branches at septum number
  one side-branch at septum
• anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  as thick as main part of branch

Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
2.4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8.2 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  rough of warts
or    with crystals

Anatomy of the crystals occouring in emanating hyphae
• presence shape
  acicular

Anatomy of rhizomorphs
• type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs

Ecology

• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  unknown

Tree

• plant family
  Fagaceae
• plant genus
  Quercus
• plant habitat kind
  nursery

Fungus

• family
  Tuberaceae
• fruitbodies growth habit
  hypogeous

Remarks

• public notes
  Mycorrhizal ends ochre-brown to brown, very tips whitish ochre; cells of inner mantle layers in plan view irregularly angular to slightly epidermoid; Hartig net in longitudinal and cross-section 1-3 cortical cell layers deep.

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