Russula alnijorullensis (Sing.) Sing. + Alnus

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Literature

references
  Becerra AG, Beenken L, Pritsch K, Daniele G, Schloter M, Agerer R (2005). Morphological description and molecular characterization of Lactarius aff. omphaliiformis, Russula alnijorullensis and Cortinarius tucumanensis ectomycorrhizas on Alnus acuminata. Mycologia 97:1047-1057

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
27 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate [irregularly]
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  abundant, dense
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
exploration type
  contact
Morphology of the unramified ends
shape
  sinuous
or    tortuous
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
10.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.1 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  yellowish [light yellow to pinkish]
older parts colour
  ochre, yellowish brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  smooth
or    covered with soil particles
emanating hyphae presence
  present
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  emanating hyphae
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  present
Anatomical features of the mantle
completeness
  complete
matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
organisation {if pseudoparenchymatous} cell shape
  angular
mantle type
  angular cells, bearing mounds of roundish cells (type K)
mound cells diameter
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mound cells length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
21 µm    Upper value of unspecified range (could be µ+s.d., but not known)
clavate end cell cover presence
  absent
hyphae arrangement
  not specific
septa clamps presence
  absent
cell pigment location-colour
  membranaceously yellowish
cell diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
19 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
32 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell density
6     Lower value of unspecified range (could be µ-s.d., but not known)
7     Upper value of unspecified range (could be µ+s.d., but not known)
cell wall with globular thickenings
  absent
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
roundish plasmatically yellow cells presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  absent
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  without pattern
hyphae vessel-like presence
  absent
septa clamps presence
  absent
cell pigment location-colour
  membranaceously yellowish
cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle different layers presence
  discernable
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
7.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
27 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer presence
  absent
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  absent

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  rectangular
or    round
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
47 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
11 µm    Lower value of unspecified range (could be µ-s.d., but not known)
22 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  paraepidermal
structure {in plan view}
  infrequently lobed
lobes width
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  tortuous
or    irregularly inflated or even beaded
cell pigment location-colour
  absent
drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
or    with ellbow-like protrusions
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell length
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
32 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    with soil particles


Anatomy of the warts occouring on emanating hyphae
presence
  absent
Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Chemical reactions

reaction with cotton-blue-lactic-acid presence
  present [emanating hyphae slightly blue]
reaction with ethanol 70% presence
  present [whole mycorrhiza bleaching]
reaction with KOH 10% presence
  present [whole mycorrhiza bleaching]
reaction with lactic acid presence
  present [whole mycorrhiza bleaching]
reaction with Melzer's reagent presence
  absent
reaction with NH4OH
  present [whole mycorrhiza bleaching]
reaction with sulpho-vanillin presence
  present [walls red, contents of mantle cells with droplets becoming black]

Ecology

geographic occurrence continent
  South America

Tree

plant family
  Betulaceae
plant genus
  Alnus

Fungus

family
  Russulaceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
380     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
320     Mean (= average)

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