Elaphomyces muricatus Fr. + Fagus

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– Enlarged view –

Literature

• references
  Brand F (1991) Ektomykorrhizen an Fagus sylvatica. Charakterisierung und Identifizierung, ökologische Kennzeichnung und unsterile Kultivierung. Libri Botanici 2: 1-229.
Brand F (1991) Elaphomyces muricatus. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 53, Einhorn-Verlag, Schwäbisch Gmünd.
Fontana A, Centrella E (1967) Ectomicorrize prodotte da funghi ipogei. Allionia 13: 149-176.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  abundant, dense
• main axis diameter
0.3 mm    Mean (= average)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  absent
• exploration type
  short distance
Morphology of the unramified ends
• shape
  bent
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.25 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown
or    ochre, yellowish brown
or    yellow
• older parts colour
  brown
or    ochre, yellowish brown
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  smooth
or    covered with soil particles
or    not smooth
• mantle surface {in detail} kind
  loosely cottony
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
• matrix presence
  present
• matrix location
  middle mantle layer
or    inner mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
• hyphal system kind
  undifferentiated
• hyphae hyphal junctions angle {between}
  ca. 45° and less
and    ca. 90°
• septa thickness {relative to cell walls}
  as thick as walls
and    thicker than walls
• septa clamps presence
  absent
• cell shape
  cylindric, not constricted at septa
• cell pigment location-colour
  absent
• cell diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
10 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
and    with few soil particles
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• matrix kind
  gelatinous
• hyphae arrangement
  plectenchymatous, without pattern
• cell pigment location-colour
  membranaceously yellowish
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• matrix kind
  gelatinous
• hyphae arrangement
  without pattern
• septa clamps presence
  present
• cell pigment location-colour
  membranaceously yellowish
• cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle thickness {at ectomycorrhizal tip}
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  not discernable
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
or    pseudoparenchymatous
• inner mantle layer organisation
  plectenchymatous
or    pseudoparenchymatous

Anatomy of the tannin cells in longitudinal section
• presence
  absent

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  round
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
or    radially-oval to -elliptic, oriented obliquely
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
19 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
9 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.3     Minimum value
0.5     Mean (= average)
1.3     Maximum value

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
Anatomy of the mantle in cross-section
• mantle different layers presence
  not discernible
• outer mantle layer organisation
  plectenchymatous
• middle mantle layer organisation
  plectenchymatous
• inner mantle layer organisation
  plectenchymatous
• unlayered mantle hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  absent

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    radially-oval to -elliptic
or    tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
11 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
0.8     Minimum value
1.1     Mean (= average)
2     Maximum value

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  one or half a row of cortical cells adjoining endodermis free of Hartig net
or    in tow rows, not reaching endodermis
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• lobes width
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• structure {in plan view}
  of palmetti type
Anatomy of the emanating elements in general
• anastomoses type
  open, with a long bridge
or    closed by a simple septum, with a long bridge
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  absent
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  approximately 90°
• distance {from septum}
  one or two hyphal diam. below the septum

Anatomy of the septa of emanating hyphae
• clamps presence
  absent


Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  with ellbow-like protrusions
• anatomy emanating elements emanating hyphae cell diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
80 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    with spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Mean (= average)
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
• type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent
Anatomy of nuclei
• number {per cell}
1     Mean (= average)
3     Maximum value
• shape
  round
• diameter
1 µm    Minimum value
2 µm    Mean (= average)
• length
1 µm    Minimum value
2 µm    Mean (= average)

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present

Chemical reactions

• reaction with acid fuchsin presence
  absent
• reaction with anilin presence
  absent
• reaction with cotton-blue-lactic-acid presence
  absent
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with KOH 10% presence
  absent
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with ruthenium-red presence
  present
• reaction with sudan III presence
  present
• reaction with sulpho-vanillin presence
  absent

Ecology

• substrate
  in organic layer
or    in organically enriched mineral horizon
• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  known

Tree

• plant family
  Fagaceae
• plant genus
  Fagus
• plant habitat kind
  forests, woods

Fungus

• family
  Elaphomycetaceae
• fruitbodies growth habit
  hypogeous

Remarks

• public notes
  Autofluorescence of mantle in section with UV-filter showing slightly creme hyphae, spindle-like cell appositions light ochre; mantle in sudan III with spindle-shaped hyphae slightly reddish, in ruthenium-red walls slightly red.

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