Lactarius vellereus (Fr.) Fr. + Fagus

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Literature

references
  Brand F (1991) Ektomykorrhizen an Fagus sylvatica. Charakterisierung und Identifizierung, ökologische Kennzeichnung und unsterile Kultivierung. Libri Botanici 2: 1-229.
Brand F, Agerer R (1986) Studien an Ektomykorrhizen VIII. Die Mykorrhizen von Lactarius subdulcis, Lactarius vellereus und Laccaria amethystina an Buche. Z Mykol 52(2): 287-320.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
40 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
tips {per 10 mm} number
12     Minimum value
38     Lower value of unspecified range (could be µ-s.d., but not known)
42     Upper value of unspecified range (could be µ+s.d., but not known)
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
2     Maximum value
main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent
Morphology of the unramified ends
shape
  straight
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
4 mm    Maximum value
diameter
0.25 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.35 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brown
or    ochre, yellowish brown
very tip colour
  whitish
older parts colour
  brown
or    red
or    grey
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle laticifers visibility
  present
mantle secreted latex {after scratching mantle} presence
  present
mantle secreted latex {after scratching mantle} colour-habit
  white, remaining white after some minutes
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  shiny
or    smooth
emanating hyphae presence
  absent
or    present
emanating hyphae abundance
  infrequent
Morphology of rhizomorphs
cross-section shape
  round or roundish
colour
  whitish
ramification kind-frequency
  infrequently, at restricted points
connection to mantle kind
  distinct
origin location
  not specific
or    proximal
margin habit
  smooth
dimorphism presence
  absent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
or    present
location
  middle mantle layer
or    inner mantle layer
shape
  straight and even
ramification presence-abundance
  infrequent
cell diameter
3 µm    Minimum value
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomical features of the mantle
matrix presence
  present
matrix location
  outer mantle layer {apart from tip}
or    outer mantle layer {of ectomycorrhizal tip}
or    middle mantle layer
or    inner mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
organisation {if pseudoparenchymatous} cell shape
  epidermoid, puzzle-like, jig-saw-shaped
mantle type
  epidermoid cells (type M)
matrix kind
  gelatinous
pores between cells presence
  present
pores between cells abundance
  infrequent
septa thickness {relative to cell walls}
  thinner than walls
septa clamps presence
  absent
cell pigment location-colour
  absent
cell density
5.7     Mean (= average)
cell wall thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
cell wall surface habit
  smooth
cell wall projections presence
  present
cell wall projections abundance
  abundant
cell wall projections shape
  even in thickness
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  pseudoparenchymatous
matrix kind
  not gelatinous
cell pigment location-colour
  membranaceously brownish
cell contents presence-kind
  absent
cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
matrix kind
  not gelatinous
hyphae arrangement
  without pattern
septa clamps presence
  absent
cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  not gelatinous
or    gelatinous
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
35 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle different layers presence
  discernable
outer mantle layer organisation
  pseudoparenchymatous
outer mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer organisation
  pseudoparenchymatous
middle mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  absent


Anatomy of the mantle in cross-section
mantle different layers presence
  discernible
outer mantle layer organisation
  pseudoparenchymatous
outer mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer organisation
  plectenchymatous
or    pseudoparenchymatous
middle mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value
inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
9 µm    Maximum value

Anatomy of the tannin cells in cross-section
presence
  absent


Anatomy of the Hartig net in cross-section
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  cylindrical
or    beaded
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
lobes width
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
structure {in plan view}
  infrequently lobed
Anatomy of the emanating elements in general
intrahyphal hyphae presence
  absent
backwards-oriented clamps presence
  absent
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  acute
distance {from septum}
  adjacent to septum
side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
clamps presence
  absent
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)


Anatomy of rhizomorphs
type
  undifferentiated; margins rather smooth; hyphae compactly arranged and of uniform diameter
(type B)
nodia presence
  absent
gelatinized hyphae presence
  absent
cup-like structures on surface presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent
Anatomy of nuclei
number {per cell}
2     Mean (= average)
shape
  round
diameter
2 µm    Mean (= average)
length
2 µm    Mean (= average)
distance {between each other}
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present

Chemical reactions

reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  present
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  absent
reaction with KOH 10% presence
  present
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with ruthenium-red presence
  present
reaction with sudan III presence
  present
reaction with sulpho-vanillin presence
  present
reaction with toluidin blue presence
  present

Ecology

geographic occurrence continent
  Europe

Tree

plant family
  Fagaceae
plant genus
  Fagus
plant habitat kind
  forests, woods

Fungus

family
  Russulaceae
subgenus-section
  Lactarius sect. Lactarius
fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

public notes
  Mycorrhizal systems mostly pyramidal; mycorrhizal ends slightly brownish to nut-brown, older parts flesh-coloured to red-brwon then darker grey-brown to grey; middle mantle layers in plan view with slightly membranaceously brownish cells; septa of laticifers infrequent; hyphae of very tip (outer mantle layers) with 1-2 um thick lumen, walls 2.5-4 um thick; rhizomorph hyphae with 1-2 um thick walls close to mantle; in cross-section Hartig net 1-2(-3) cortical cell layers deep, in older parts protruding towards endodermis; autofluorescence of mantle in section with UV-filter outer layers distisnctly light blue and middle mantle layers olive-yellow and inner mantle layers slightly blue, with blue-filter greenish yellow and middle mantle layers rather greenith orange; mantle in sulfo-vanillin partly slightly violet-brown, latex without reaction, in KOH latex grey, in brillant-cresyl-blue violet-blue, in toluidin-blue blue-violet, in cotton-blue walls of outer mantle layers distinctly blue, in sudan III latex distinctly red-orange, in ruthenium-red walls of outer mantle layer red, in chlorazol black slightly blue-green, in phloxin B brown-red; (after Brand (1991) possibly misidentified, this mycorrhiza was also found associated with L. blennius!).

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