"Pinirhiza lactariosimilis" + Pinus

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Literature

references
  Golldack J, Münzenberger B, Hüttl RF (1997) "Pinirhiza lactariosimilis" + Pinus sylvestris L. Descr Ectomyc 2: 43-48.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  dichotomous
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
Morphology of the unramified ends
shape
  straight
or    bent
shape {of distal end}
  not inflated, cylindric
or    inflated, club-shaped
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
2.7 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  brown
or    ochre, yellowish brown
or    yellow
very tip colour
  whitish
older parts colour
  dark brown
or    ochre, yellowish brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  shiny
or    smooth

Anatomy

Anatomical features of the entire mycorrhiza
Anatomy of laticifers
presence
  absent
or    present
location
  middle mantle layer
or    inner mantle layer
shape
  straight and even
ramification presence-abundance
  infrequent
cell diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
organisation {if pseudoparenchymatous} cell shape
  epidermoid, puzzle-like, jig-saw-shaped
mantle type
  epidermoid cells bearing a hyphal net (type Q)
hyphal net cells shape
  not specialized
hyphal net cells diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
hyphal net cell walls habit
  distinct, rather thick
septa clamps presence
  absent
cell pigment location-colour
  absent
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
27 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2 µm    Maximum value
cell wall surface habit
  smooth
cell wall projections presence
  present
cell wall projections abundance
  infrequent
cell wall projections shape
  thicker at distal end
Anatomy of the middle mantle layer
organisation
  plectenchymatous
or    pseudoparenchymatous
hyphae arrangement
  plectenchymatous, without pattern
cell pigment location-colour
  membranaceously yellowish
cell diameter
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
cell length
9 µm    Minimum value
12 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
35 µm    Maximum value
cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2 µm    Maximum value
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  without pattern
or    ring-like
septa clamps presence
  absent
cell pigment location-colour
  membranaceously yellowish
cell diameter
1.5 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
cell length
8 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
37 µm    Upper value of unspecified range (could be µ+s.d., but not known)
70 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
22 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
mantle thickness {at ectomycorrhizal tip}
21 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
mantle different layers presence
  discernable
outer mantle layer hyphae tangentially length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
19 µm    Maximum value
outer mantle layer hyphae radially diameter
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
middle mantle layer hyphae tangentially length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
55 µm    Maximum value
middle mantle layer hyphae radially diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer hyphae tangentially length
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
inner mantle layer hyphae radially diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
1     Mean (= average)
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
mean tangenial length TCt
42.4 µm    Mean (= average)
mean shape-ratio TCq
4.6     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  round
or    radially-oval to -elliptic
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
29 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.54     Mean (= average)

Anatomy of the Hartig net in longitudinal section
anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
backwards-oriented clamps presence
  absent
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  straight
drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
clamps presence
  absent
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall thickness
1 µm    Mean (= average)


Anatomy of rhizomorphs
type
  lacking, only emanating hyphae present
(type G)

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present

Chemical reactions

reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with sulpho-vanillin presence
  present

Ecology

geographic occurrence continent
  Europe

Tree

plant family
  Pinaceae
plant genus
  Pinus
plant subgenus-section
  two-needle-pines
plant habitat kind
  forests, woods

Remarks

public notes
  Mycorrhizal ends more often not inflated at tip, ochre with yellowish brown patches, older parts dark brown with yellowish-brown patches; hyphal net of mantle surface in plan view with 8-30 um long cells; mantle surface with sticky matrix; middle mantle layers a transitional type between plectenchymatous and pseudoparenchymatous with partially epidermoid cells; inner mantle layers in plan view only occasionally with ring-like hyphal arrangement; autofluorescence of mantle in section with UV-filter yellowish green; mantle in sulfo-vanillin with greyish brown laticifers.

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