Ramaria spinulosa (Fr.) Quel. + Fagus

– Item listing –

– Enlarged view –

Literature

references
  Agerer R (1996) Ramaria spinulosa (Fr.) Quel. + Fagus sylvatica L. Descr Ectomyc 1: 119-124.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
6.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  nest-like, hyphal mats
main axis diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.36 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  present
rhizomorphs presence
  present
rhizomorphs frequency
  abundant
exploration type
  medium distance mat
Morphology of the unramified ends
shape
  straight
or    bent
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1.2 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.21 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.24 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  white
very tip colour
  yellowish
older parts colour
  black
or    grey
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
or    not smooth
mantle surface {in detail} kind
  densely stringy
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  not specifically distributed
Morphology of rhizomorphs
diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.12 mm    Upper value of unspecified range (could be µ+s.d., but not known)
2 mm    Maximum value
cross-section shape
  round or roundish
or    flat
colour
  white
ramification kind-frequency
  repeatedly into smaller filaments
connection to mantle kind
  oblique
origin location
  not specific
or    proximal
margin habit
  hairy
dimorphism presence
  absent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    cystidia
emanating elements cystidia location
  on outer mantle layer
or    on rhizomorphs
crystals presence
  present
crystals location
  emanating hyphae
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
matrix presence
  present
matrix location
  outer mantle layer {apart from tip}
or    outer mantle layer {of ectomycorrhizal tip}
or    middle mantle layer
or    inner mantle layer
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
matrix kind
  not gelatinous
hyphal system kind
  undifferentiated
septa thickness {relative to cell walls}
  as thick as walls
and    thicker than walls
septa clamps presence
  absent
cell shape
  cylindric, constricted at septa
and    slightly inflated in middle portions
and    ampullate at both sides of septum
cell pigment location-colour
  absent
cell contents presence-kind
  absent
cell diameter
3.5 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
cell length
75 µm    Lower value of unspecified range (could be µ-s.d., but not known)
135 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.2 µm    Mean (= average)
cell wall surface habit
  rough
and    with few soil particles
drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
organisation
  plectenchymatous
matrix kind
  not gelatinous
or    gelatinous
hyphae arrangement
  plectenchymatous, without pattern
cell pigment location-colour
  colourless
cell diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
70 µm    Maximum value
cell contents presence-kind
  absent
cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
matrix kind
  not gelatinous
or    gelatinous
hyphae arrangement
  without pattern
septa clamps presence
  absent
cell pigment location-colour
  absent
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  plectenchymatous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  gelatinous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
mantle thickness {at ectomycorrhizal tip}
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle different layers presence
  not discernable
outer mantle layer organisation
  plectenchymatous
middle mantle layer organisation
  plectenchymatous
inner mantle layer organisation
  plectenchymatous
unlayered mantle hyphae tangentially length
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
unlayered mantle hyphae radially diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  absent

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
or    radially-oval to -elliptic, oriented obliquely

Anatomy of the Hartig net in longitudinal section
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type
lobes width
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
Anatomy of the mantle in cross-section
mantle different layers presence
  not discernible
outer mantle layer organisation
  plectenchymatous
middle mantle layer organisation
  plectenchymatous
inner mantle layer organisation
  plectenchymatous
unlayered mantle hyphae tangentially length
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
unlayered mantle hyphae radially diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in cross-section
presence
  absent

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  round
or    radially-oval to -elliptic
or    tangentially-oval to tangentially-elliptic

Anatomy of the emanating elements in general
intrahyphal hyphae presence
  present
septal pores configuration
  globular thickenings
backwards-oriented ramifications presence
  present
backwards-oriented clamps presence
  absent
anastomoses type
  open, with a short bridge or bridge almost lacking
anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
anastomoses location
  not specified
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  as acanthocystidia (type P)
septa presence
  absent
septa kind
  absent, with basal clamp only
diameter {proximal}
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
80 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall colour
  absent
cell wall colour {relative to mantle cells}
  darker
cell wall thickness
0.2 µm    Mean (= average)
cell wall thickness {relative to mantle cells}
  thinner
cell wall evenness
  even in thickness
surface habit
  smooth
contents presence
  present
contents type
  finely granular

Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  absent
drops of exuded pigment presence
  present

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  acute
distance {from septum}
  adjacent to septum
or    one or two hyphal diam. below the septum
side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
clamps presence
  absent
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  slightly constricted
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell length
35 µm    Lower value of unspecified range (could be µ-s.d., but not known)
110 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  rough of warts
or    with crystals
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall surface structures shape
  hemispherical warts
anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Mean (= average)
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness

Anatomy of the crystals occouring in emanating hyphae
presence shape
  cuboid
or    octahedral
orientation
  irregularly
length
0.5 µm    Minimum value
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of rhizomorphs
type
  slightly differentiated; central hyphae somewhat enlarged
(type C)
nodia presence
  absent
internal nodia presence
  absent
gelatinous matrix presence
  present
gelatinized hyphae presence
  absent
cup-like structures on surface presence
  absent
conical young side-branches presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
contents presence-kind
  filled with brownish or yellowish substances
ampullate, trumpet-like inflated presence
  present
ampullate inflations thickness
5 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
5 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
5 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
5 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  filled with brownish or yellowish substances
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  present
anatomy emanating elements rhizomorphs hyphae ampullate inflations thickness
5 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
11 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent
Anatomy of nuclei
number {per cell}
2     Mean (= average)
shape
  round
or    oval
diameter
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
1.5 µm    Mean (= average)
distance {between each other}
0 µm    Mean (= average)

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present
mantle in section blue-filter, 450-490 nm presence
  present
mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

reaction with anilin presence
  absent
reaction with cotton-blue-lactic-acid presence
  present
reaction with ethanol 70% presence
  present
reaction with FeSO4 presence
  present
reaction with formol 40% presence
  present
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  present
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with phenole presence
  absent
reaction with phenole-aniline presence
  absent
reaction with sulpho-vanillin presence
  absent

Ecology

substrate
  in mineral soil
geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Fagaceae
plant genus
  Fagus
plant habitat kind
  forests, woods

Fungus

family
  Ramariaceae
fruitbodies growth habit
  epigeous
or    clavarioid

Remarks

public notes
  Older parts of mycorrhizal ends greyish and becoming black with age; outer mantle layers in plan view with rough hyphae near rhizomorph connection; cystidia infrequent, preferrably occurring on rhizomorphs, sometimes with globular inflations up to 6 um in diam., outgrowths 4-6x1 um; emanating hyphae near proximal ends up to 5.5 um thick, crystals very infrequent; rhizomorph central hyphae rarely with slightly thicker septa than walls, cell walls of inflations 0.5-1 um thick; peripheral hyphae crystals 1.5-5 um thick; in longitudinal section Hartig net 1-2(-3) cortical cell layers deep; autofluorescence of mantle in section with UV-filter distinctly blue, with blue-filter distinctly green, with green-filter red; mantle in KOH yellowish, in FeSO4 greenish, in lactic acid crystals and warts of cell walls not dissolving, in formol greenish, in cotton-blue walls slightly blue, in ethanol slightly brownish, in safranine walls red.

– Item listing –