Descomyces albus (Klotzsch) Bougher & Castellano + Eucalyptus

– Item listing –

Literature

references
  Agerer R, Beenken L, Bougher NL (2001) Descomyces albus (Klotzsch) Bougher & Castellano + Eucalyptus spec. Descr Ectomyc 5: 41-47.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
10 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
tips {per 10 mm} number
8     Lower value of unspecified range (could be µ-s.d., but not known)
17     Upper value of unspecified range (could be µ+s.d., but not known)
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Upper value of unspecified range (could be µ+s.d., but not known)
2     Maximum value
abundance
  abundant, dense
main axis diameter
0.15 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.15 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.15 mm    Mean (= average)
taste {after 2-3 min chewing}
  unknown
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  absent
exploration type
  short distance
Morphology of the unramified ends
mantle hydrophobicity presence
  absent
shape
  bent [slightly]
or    sinuous [slightly]
shape {of distal end}
  not inflated, cylindric
or    tapering [slightly]
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
2 mm    Maximum value
diameter
0.12 mm    Minimum value
0.15 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.15 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.18 mm    Maximum value
colour
  brown [greyish brown]
or    greyish [greyish brown]
very tip colour
  whitish
after injury colour
  unchanged
older parts colour
  brown
or    ochre, yellowish brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  not smooth
mantle surface {in detail} kind
  loosely woolly
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  not specifically distributed
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  emanating hyphae
or    cystidia
emanating elements cystidia location
  on outer mantle layer
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  absent
Anatomical features of the mantle
completeness
  complete
matrix presence
  unknown
matrix location
  unknown
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae arranged net-like, with prominent cystidia (type D)
matrix kind
  unknown
hyphae short protrusions presence
  present
hyphae sticky matrix on hyphal surface presence
  unknown
hyphae hyphal junctions angle {between}
  unknown
septa thickness {relative to cell walls}
  as thick as walls
septa clamps presence
  present
septa clamps abundance
  abundant
cell shape
  cylindric, not constricted at septa
cell pigment location-colour
  absent
cell contents presence-kind
  absent
cell diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
  variable
cell wall thickness
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Mean (= average)
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  present
organisation
  plectenchymatous [sometimes appearing pseudoparenchymatous]
matrix kind
  unknown
hyphae arrangement
  plectenchymatous, without pattern
cell pigment location-colour
  colourless
cell diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
cell length
  unknown
cell contents presence-kind
  absent
cell wall thickness
0.1 µm    Mean (= average)
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
matrix kind
  unknown
hyphae arrangement
  without pattern
septa thickness
  unknown
septa clamps presence
  absent
cell pigment location-colour
  absent
cell diameter
2 µm    Minimum value
3.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
  unknown
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  not gelatinous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
  unknown
calyptra cell remnants presence
  unknown
calyptra cell remnants type
  unknown
mantle different layers presence
  not discernable
unlayered mantle organization
  plectenchymatous
unlayered mantle hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
unlayered mantle hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  absent

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic, oriented obliquely
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
19 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
37 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
11.3 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.25     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  paraepidermal
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish
or    cylindrical
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type [weakly ramified]
lobes width
0.5 µm    Minimum value
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae presence
  unknown
intrahyphal hyphae clamps presence
  unknown
septal pores configuration
  unknown
backwards-oriented ramifications presence
  unknown
backwards-oriented clamps presence
  unknown
anastomoses type
  closed by a simple septum, with a short bridge or bridge almost lacking (contact-septum)
anastomoses surface habit
  smooth
anastomoses cell wall thickness {relative to remaining cell walls}
  unknown
anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
anastomoses location
  not specified
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  lateral, with tapering, knob-bearing, capitate outgrowth (type M)
apical knobs number
1     Mean (= average)
apical knobs arrangement
  symmetrically
apical knobs shape
  globose
apical knobs diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
apical knobs length
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
apical knobs wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1 µm    Maximum value
apical knobs cell wall thickness {relative to cystidial body}
  unknown
ramification presence-position
  absent
septa presence
  present [sometimes below the globule]
septa kind
  simple
septa distance {between}
  unknown
septa number
0     Lower value of unspecified range (could be µ-s.d., but not known)
1     Maximum value
basal clamp walls abruptly thick
  absent
foot cell presence
  not applicable
diameter {proximal}
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter {distal}
  variable [lateral outgrowth (4)6-11(15) µm long, distally 1(1,5) µm wide, proximally (1,5)2-2,5 µm diam.]
length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
22 µm    Upper value of unspecified range (could be µ+s.d., but not known)
31 µm    Maximum value
cell wall colour
  absent
cell wall colour {relative to mantle cells}
  unknown
cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness {relative to mantle cells}
  unknown
cell wall evenness
  unknown
surface habit
  smooth
contents presence
  absent

Anatomy of emanating hyphae
shape
  straight
or    wavy
cell pigment location-colour
  membranaceously yellowish
pigment presence-distribution
  only lacking at tips
cell contents presence-kind
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  acute
distance {from septum}
  unknown
side-branches at septum number
  unknown
anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  as thick as main part of branch

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps surface kind
  smooth
clamps outline {in dorsal view}
  cylindric
clamps width {relative to hypha in dorsal view}
  as broad as
clamps outline {in lateral view}
  as a semicircle
or    more than a semicircle
or    not constricted at contact point to subtending hyphal cell
or    constricted at contact point to subtending hyphal cell
clamps width {relative to hypha in lateral view}
  as broad as
clamps hole presence
  absent
clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  present
abundance
  infrequent [very]
distribution
  unknown

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
or    tortuous, screw-like
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell length
85 µm    Minimum value
110 µm    Lower value of unspecified range (could be µ-s.d., but not known)
160 µm    Upper value of unspecified range (could be µ+s.d., but not known)
190 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Mean (= average)
anatomy emanating elements emanating hyphae cell wall layers number
  unknown
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  thinner
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present [slightly bluish, particularly thick-walled portions of cystidia]
mantle in section blue-filter, 450-490 nm presence
  present [slightly greenish, particularly thick-walled portions of cystidia]
mantle in section green-filter, 530-560 nm presence
  absent

Chemical reactions

reaction with brillant-cresyl-blue presence
  present [slightly blue]
reaction with FeSO4 presence
  present [mantle hyphae and tips of emanating hyphae slightly blue]
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  present [15%: mantle slightly brownish, emanating hyphae turning from yellow to brown]
reaction with lactic acid presence
  absent
reaction with Melzer's reagent presence
  amyloid
amyloidity or dextrinoidity location
  patches of hyphal walls
or    septa, globular thickenings of septa
reaction with sulpho-vanillin presence
  absent
reaction with water presence
  colour changing [freshly isolated mycorrhizae become bluish during preparation]

Ecology

substrate
  in organically enriched mineral horizon
geographic occurrence continent
  Australia
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Myrtaceae
plant genus
  Eucalyptus
plant habitat kind
  forests, woods

Fungus

family
  Bolbitiaceae
fruitbodies growth habit
  hypogeous
or    gastroid

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme Alu bands number
4     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
370     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
130     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
120     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 4. band from below number
105     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
350     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
325     Mean (= average)

Remarks

public notes
  EcoRI: 2x 360 bp; TaqI: 340 bp, 170 bp, 70 bp

– Item listing –