Russula alnetorum Romagn. + Alnus

– Item listing –

Literature

references
  Beenken L (2001) Russula alnetorum Romagn. + Alnus viridis (Chaix) DC. Descr Ectomyc 5: 115-123.
Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, Universität München. http://edoc.ub.uni-muenchen.de/archive/00003175/01/Beenken_Ludwig.pdf

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
12 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  absent
or    monopodial-pinnate [rarely, mostly simple]
tips {per 10 mm} number
  unknown
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  unknown
main axis diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
taste {after 2-3 min chewing}
  unknown
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent [very]
exploration type
  medium distance smooth
Morphology of the unramified ends
mantle hydrophobicity presence
  absent
shape
  straight
or    bent [slightly]
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  yellowish
very tip colour
  unknown
after injury colour
  unchanged
older parts colour
  ochre, yellowish brown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  unknown
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  whitish [occasionally]
mantle carbonizing presence
  absent
mantle surface {in general} habit
  not smooth
mantle surface {in detail} kind
  loosely grainy or warty
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  unknown
Morphology of rhizomorphs
diameter
0.2 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  cushion-shaped (semicircular)
colour
  ochre, yellowish brown
after injury colour
  unknown
ramification kind-frequency
  infrequently, at restricted points
connection to mantle kind
  distinct
origin location
  proximal
connection to substrate
  almost consistently growing along roots
margin habit
  hairy
dimorphism presence
  unknown
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    emanating hyphae
emanating elements cystidia location
  not applicable
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  present
Anatomical features of the mantle
completeness
  complete
matrix presence
  present
matrix location
  surface net [on mantle surface]
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
organisation {if pseudoparenchymatous} cell shape
  angular
mantle type
  angular cells, bearing mounds of roundish cells (type K)
and    angular cells and mounds of flattened cells (type O)
mound cells shape
  triangular [a second type solitary, irreguarly elongate]
and    ovoid to cylindric
mound cells diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mound cells length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
26 µm    Maximum value
mound cells cell wall thickness
0.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mound cells cell wall kind
  smooth [verrucose, contents with droplets]
mound cells granular contents
  absent
clavate end cell cover presence
  absent
matrix kind
  gelatinous
hyphae arrangement
  not specific
hyphae sticky matrix on hyphal surface presence
  present
pores between cells presence
  unknown
septa thickness {relative to cell walls}
  unknown
septa clamps presence
  absent
cell pigment location-colour
  membranaceously yellowish
cell contents presence-kind
  droplets, that stain in sulpho-vanillin
cell diameter
3 µm    Minimum value
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
cell wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1 µm    Maximum value
cell wall with globular thickenings
  absent
cell wall surface habit
  smooth
cell wall projections presence
  unknown
cell wall projections abundance
  unknown
cell wall projections shape
  unknown
drops of exuded pigment presence
  absent
roundish plasmatically yellow cells presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  present
organisation
  plectenchymatous [inner portions]
or    pseudoparenchymatous [outer portions]
matrix kind
  not applicable
hyphae arrangement
  plectenchymatous, without pattern
cell shape
  polygonal
cell pigment location-colour
  membranaceously yellowish
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
cell wall thickness
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Mean (= average)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous [densely]
matrix kind
  not applicable
hyphae arrangement
  without pattern
hyphae vessel-like presence
  absent
septa thickness
  unknown
septa clamps presence
  absent
cell pigment location-colour
  membranaceously yellowish
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  with oily droplets, which do not stain in sulpho-vanillin
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  pseudoparenchymatous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
10 µm    Mean (= average)
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
calyptra cell remnants presence
  present
calyptra cell remnants type
  in two rows [up to three]
mantle different layers presence
  not discernable
unlayered mantle organization
  unknown
unlayered mantle hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
unlayered mantle hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  absent

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  radially-oval to -elliptic, oriented obliquely
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  paraepidermal
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  cylindrical
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type
lobes width
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
3 µm    Maximum value
lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae presence
  present
intrahyphal hyphae clamps presence
  absent
septal pores configuration
  unknown
backwards-oriented ramifications presence
  unknown
backwards-oriented clamps presence
  absent
anastomoses type
  unknown
Anatomy of cystidia


Anatomy of emanating hyphae
cell pigment location-colour
  absent
or    membranaceously yellowish
pigment presence-distribution
  unknown
cell contents presence-kind
  absent
drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
1 µm    Maximum value


Anatomy of the warts occouring on emanating hyphae
presence
  absent
Anatomy of rhizomorphs
type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
nodia presence
  absent
internal nodia presence
  absent
gelatinous matrix presence
  present
gelatinized hyphae presence
  absent
hyphae distinctly different diameter
  absent
cup-like structures on surface presence
  absent
conical young side-branches presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
contents presence-kind
  absent
ampullate, trumpet-like inflated presence
  absent
roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present [yellow-green throughout]
mantle in section blue-filter, 450-490 nm presence
  present [yellow throughout]
mantle in section green-filter, 530-560 nm presence
  present [red throughout]

Chemical reactions

reaction with acetic acid presence
  present [walls slightly pink]
reaction with cotton-blue-lactic-acid presence
  present [walls blue]
reaction with ethanol 70% presence
  present [droplets dissolved]
reaction with FeSO4 presence
  present [mantle olive]
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  present [droplets dissovled]
reaction with lactic acid presence
  present [droplets dissloved]
reaction with Melzer's reagent presence
  absent
reaction with sulpho-vanillin presence
  present [walls pink, contents of mantle cells with droplets becoming light brown]

Ecology

substrate
  in mineral soil
soil pH
  acid reaction
geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Betulaceae
plant genus
  Alnus
plant habitat kind
  forests, woods

Fungus

family
  Russulaceae
fruitbodies growth habit
  epigeous
or    pileate-smooth or wrinkled

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme Alu bands number
3     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
500     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
240     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
50     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
370     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
330     Mean (= average)

Remarks

public notes
  EcoRI: 390 bp, 320 bp, 50 bp, 40 bp; TaqI: 380 bp, 275 bp, 60 bp

– Item listing –