Amanita muscaria (L. : Fr.) Hooker + Pinus

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Literature

references
  Ingleby K, Mason PA, Last FT, Fleming LV (1990) Identification of ectomycorrhizas. ITE Res Publ 5, HSMO, London.
Mohan V, Natarajan K, Ingleby K (1995) Anatomical studies on ectomycorrhizas II. The ectomycorrhizas produced by Amanita muscaria, Laccaria laccata and Suillus brevipes on Pinus patula. Mycorrhiza 3(1): 43-49.
Raidl S (1997) Studien zur Ontogenie an Rhizomorphen von Ektomykorrhizen. Bibl Mycol 169: 1-184.
Uhl M (1988) Charakterisierung und Identifizierung von Ektomykorrhizen an Pinus silvestris und von Ektomykorrhizen aus der Gattung Tricholoma. Diss Uni München.

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  dichotomous
main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent
exploration type
  medium distance smooth
Morphology of the unramified ends
shape
  straight
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  white
very tip colour
  white
older parts colour
  ochre, yellowish brown
or    yellow
or    grey
or    white
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  absent
mantle carbonizing presence
  absent
mantle surface {in general} habit
  silvery
emanating hyphae presence
  absent
Morphology of rhizomorphs
diameter
0.05 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.125 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  round or roundish
colour
  whitish
ramification kind-frequency
  infrequently, at restricted points
origin location
  proximal
margin habit
  smooth
dimorphism presence
  absent
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  plectenchymatous
mantle type
  hyphae arranged net-like, repeatedly and squarrosely branched (type E)
hyphae hyphal junctions angle {between}
  ca. 120°
septa clamps presence
  absent
cell shape
  cylindric, constricted at septa
cell pigment location-colour
  membranaceously yellowish
and    plasmatically yellowish
cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
Anatomy of the middle mantle layer
organisation
  plectenchymatous
hyphae arrangement
  plectenchymatous, without pattern
cell diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.5 µm    Mean (= average)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  without pattern
septa clamps presence
  absent
cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  of a transistional type between plectenchymatous and pseudoparenchymatous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle different layers presence
  discernable
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer hyphae radially diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
30 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean tangenial length TCt
51.2 µm    Mean (= average)
mean shape-ratio TCq
6.9     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  round
or    radially-oval to -elliptic
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
80 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
70 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
43.6 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
0.9     Mean (= average)

Anatomy of the Hartig net in longitudinal section
structure {in plan view}
  of palmetti type
lobes width
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section
mantle different layers presence
  discernible
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in cross-section
presence
  present
rows number
1     Mean (= average)
shape
  tangentially-oval to tangentially-elliptic
tangentially length
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
50 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean tangential length TCt
38.5 µm    Mean (= average)
mean shape-ratio TCq
5.2     Mean (= average)

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  round
anatomy mantle cross-section cortical (epidermal) cells tangentially length
25 µm    Lower value of unspecified range (could be µ-s.d., but not known)
65 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section cortical (epidermal) cells radially diameter
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
70 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
43 µm    Mean (= average)
anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
0.9     Mean (= average)

Anatomy of the Hartig net in cross-section
presence
  present
kind
  protruding towards endodermis
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
anatomy mantle cross-section hyphal cells around tannin cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
backwards-oriented ramifications presence
  present
anastomoses type
  open, with a short bridge or bridge almost lacking
Anatomy of cystidia


Anatomy of emanating hyphae
cell pigment location-colour
  membranaceously yellowish
or    plasmatically yellowish


Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Mean (= average)


Anatomy of rhizomorphs
type
  slightly differentiated; central hyphae somewhat enlarged
(type C)
or    highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
nodia presence
  present
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of nuclei
number {per cell}
2     Mean (= average)
3     Maximum value
shape
  round
or    oval
diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of siderophilous granulations
presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  absent
mantle in section blue-filter, 450-490 nm presence
  present
mantle in section green-filter, 530-560 nm presence
  absent

Chemical reactions

reaction with acid fuchsin presence
  absent
reaction with anilin presence
  absent
reaction with brillant-cresyl-blue presence
  present [blue]
reaction with cotton-blue-lactic-acid presence
  absent
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent
reaction with Melzer's reagent presence
  absent
reaction with phenole presence
  absent
reaction with phenole-aniline presence
  absent
reaction with sulpho-vanillin presence
  absent

Ecology

geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  known

Tree

plant family
  Pinaceae
plant genus
  Pinus
plant subgenus-section
  two-needle-pines
plant habitat kind
  forests, woods

Fungus

family
  Amanitaceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

Remarks

public notes
  Older parts of mycorrhizae greyish-white to yellowish-white; distal ends of emanating hyphae 3-5 um thick, often cystidia-like; autofluorescence of mantle in section with blue-filter reddish; mantle in brillant-cresyl-blue blue.

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