"Piceirhiza nigra" + Picea

– Item listing –

– Enlarged view –

Literature

• references
  Agerer R, Klostermeyer D, Steglich W (1995) Piceirhiza nigra: An ectomycorrhiza on Picea formed by a species of Thelephoraceae. New Phytol 131: 377-380.
Berg B (1989) Charakterisierung und Vergleich von Ektomykorrhizen gekalkter Fichtenbestände. Diss Univ München.
Berg B, Gronbach E (1988) Piceirhiza nigra. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 19, Einhorn-Verlag, Schwäbisch Gmünd.
Franz F (1994) Ektomykorrhizen der Fichte: Identifizierung, Ultrastruktur und Mikroelementanalyse (EELS, ESI). Diss Univ Bayreuth.
Gronbach E (1988) Charakterisierung und Identifizierung von Ektomykorrhizen in einem Fichtenbestand mit Untersuchungen zur Merkmalsvariabilität in sauer beregneten Flächen. Bibl Mycol 125: 1-216.
Haug I, Pritsch K (1992) Ectomycorrhizal types of spruce (Picea abies (L.) Karst.) in the Black Forest. A microscopical atlas. Kernforschungszentrum, Karlsruhe.
Weiss M (1988) Ektomykorrhizen von Picea abies. Synthese, Ontogenie und Reaktion auf Umweltschadstoffe. Diss Univ München.

Morphology

Morphology of the mycorrhizal system
• length
3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
16 mm    Upper value of unspecified range (could be µ+s.d., but not known)
20 mm    Maximum value
• ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  solitary or in small numbers
• main axis diameter
0.4 mm    Minimum value
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs presence
  absent
• exploration type
  short distance
Morphology of the unramified ends
• shape
  straight
or    bent
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.2 mm    Minimum value
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 mm    Maximum value
• colour
  dark brown
• very tip colour
  brown
or    brownish
• older parts colour
  black
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  brownish
or    concolorous to mantle
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  shiny
• mantle surface {in detail} kind
  densely grainy or warty
• emanating hyphae presence
  present
• emanating hyphae abundance
  infrequent
• emanating hyphae distribution
  concentrated proximally
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  cystidia
• emanating elements cystidia location
  on outer mantle layer
• blue granules presence
  present
• blue granules location
  outer mantle layer
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
• matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  pseudoparenchymatous
• organisation {if pseudoparenchymatous} cell shape
  angular
• mantle type
  angular cells, bearing mounds of roundish cells (type K)
• mound cells diameter
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mound cells granular contents
  absent
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously brownish
• cell diameter
5 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
16 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
• cell length
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
• cell density
4     Lower value of unspecified range (could be µ-s.d., but not known)
8     Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2 µm    Maximum value
• cell wall with globular thickenings
  absent
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
• blue granules location
  within cells
Anatomy of the middle mantle layer
• organisation
  pseudoparenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
• cell pigment location-colour
  membranaceously brownish
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  star-like
• septa clamps presence
  present
• cell pigment location-colour
  absent
or    membranaceously brownish
• cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell length
8 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
23 µm    Upper value of unspecified range (could be µ+s.d., but not known)
38 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
6     Lower value of unspecified range (could be µ-s.d., but not known)
9     Upper value of unspecified range (could be µ+s.d., but not known)
10     Maximum value
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
30 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
80 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle thickness {at ectomycorrhizal tip}
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• mantle different layers presence
  discernable
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
4 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• outer mantle layer hyphae radially diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• inner mantle layer hyphae radially diameter
1 µm    Minimum value
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
3     Maximum value
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
20 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
110 µm    Maximum value
• radially diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• mean tangenial length TCt
53.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
70.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean shape-ratio TCq
7.2     Lower value of unspecified range (could be µ-s.d., but not known)
7.5     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
130 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
7 µm    Minimum value
14 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
36 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
75.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
78.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
3.7     Lower value of unspecified range (could be µ-s.d., but not known)
4.4     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two
• anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• structure {in plan view}
  of palmetti type
• lobes width
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  pseudoparenchymatous
• outer mantle layer hyphae tangentially length
3 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
• outer mantle layer hyphae radially diameter
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
1.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
• inner mantle layer hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
3     Maximum value
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
20 µm    Minimum value
23 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• radially diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
• mean tangential length TCt
30.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
32.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean shape-ratio TCq
3.1     Lower value of unspecified range (could be µ-s.d., but not known)
3.4     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
12 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
65 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
7 µm    Minimum value
14 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
36 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
23.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
33.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.2     Lower value of unspecified range (could be µ-s.d., but not known)
1.8     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  present
Anatomy of cystidia

Anatomy of cystidia (type 1)
• type
  awl-shaped, bristle-like (type A)
or    bottle-shaped with a straight neck (type B)
• septa presence
  absent
or    present
• septa kind
  absent, with basal clamp only
or    simple
• diameter {proximal}
4 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter {distal}
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
10 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
54 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• cell wall colour
  brownish
• cell wall colour {relative to mantle cells}
  similar
• cell wall thickness {relative to mantle cells}
  thicker
• cell wall evenness
  even in thickness
• surface habit
  smooth
• contents presence
  absent
• contents type
  absent

Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  absent
or    membranaceously brownish
• drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  acute
or    approximately 90°
• distance {from septum}
  adjacent to septum
or    one or two hyphal diam. below the septum
or    in considerable distance from the septum
• side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps outline {in dorsal view}
  oval
• clamps width {relative to hypha in dorsal view}
  thinner than
or    as broad as
• clamps outline {in lateral view}
  less than a semicircle
or    not constricted at contact point to subtending hyphal cell
• clamps width {relative to hypha in lateral view}
  thinner than
• clamps hole presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  present
• abundance
  abundant
• distribution
  evenly distributed

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
• anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
• anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
• anatomy emanating elements emanating hyphae cell length
22 µm    Minimum value
35 µm    Lower value of unspecified range (could be µ-s.d., but not known)
130 µm    Upper value of unspecified range (could be µ+s.d., but not known)
160 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall surface structures shape
  hemispherical warts
• anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 µm    Maximum value
• anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  as thick as
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  present
• {of ectomycorrhiza former} abundance
  occasionally present
• shape
  as normal hyphae
Anatomy of nuclei
• number {per cell}
1     Minimum value
2     Mean (= average)
3     Maximum value
• shape
  round
• diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• length
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
Ultrastructure
• septal pores type
  as dolipores with perforated parenthesome

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  absent
• mantle in section blue-filter, 450-490 nm presence
  absent

Chemical reactions

• reaction with acid fuchsin presence
  absent
• reaction with anilin presence
  absent
• reaction with brillant-cresyl-blue presence
  present
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  present
• reaction with lactic acid presence
  present
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  present
• reaction with ruthenium-red presence
  absent
• reaction with sulpho-vanillin presence
  present
• chemical compounds type
  thelephoric acid

Ecology

• geographic occurrence continent
  Europe

Tree

• plant family
  Pinaceae
• plant genus
  Picea [abies]
• plant habitat kind
  forests, woods

Fungus

• family
  Thelephoraceae ss. Stalpers. excl. Tylospora

Remarks

• public notes
  Mycorrhizal ends at most slightly bent; in cross-section tannin cells also half-moon shaped; mantle in sulfo-vanillin with violet cell walls and in total brownish-green, in KOH walls greenish and in total olive, in lactic acid walls brownish-ochre, in brillant-cresyl-blue violet, in cotton-blue walls greenish-brown, in phenole walls olive-green.

– Item listing –