"Piceirhiza nigra" + Picea

– Item listing –

– Enlarged view –

Literature

references
  Agerer R, Klostermeyer D, Steglich W (1995) Piceirhiza nigra: An ectomycorrhiza on Picea formed by a species of Thelephoraceae. New Phytol 131: 377-380.
Berg B (1989) Charakterisierung und Vergleich von Ektomykorrhizen gekalkter Fichtenbestände. Diss Univ München.
Berg B, Gronbach E (1988) Piceirhiza nigra. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 19, Einhorn-Verlag, Schwäbisch Gmünd.
Franz F (1994) Ektomykorrhizen der Fichte: Identifizierung, Ultrastruktur und Mikroelementanalyse (EELS, ESI). Diss Univ Bayreuth.
Gronbach E (1988) Charakterisierung und Identifizierung von Ektomykorrhizen in einem Fichtenbestand mit Untersuchungen zur Merkmalsvariabilität in sauer beregneten Flächen. Bibl Mycol 125: 1-216.
Haug I, Pritsch K (1992) Ectomycorrhizal types of spruce (Picea abies (L.) Karst.) in the Black Forest. A microscopical atlas. Kernforschungszentrum, Karlsruhe.
Weiss M (1988) Ektomykorrhizen von Picea abies. Synthese, Ontogenie und Reaktion auf Umweltschadstoffe. Diss Univ München.

Morphology

Morphology of the mycorrhizal system
length
3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
16 mm    Upper value of unspecified range (could be µ+s.d., but not known)
20 mm    Maximum value
ramification presence-type
  monopodial-pinnate
or    monopodial-pyramidal
ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
abundance
  solitary or in small numbers
main axis diameter
0.4 mm    Minimum value
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs presence
  absent
exploration type
  short distance
Morphology of the unramified ends
shape
  straight
or    bent
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.2 mm    Minimum value
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 mm    Maximum value
colour
  dark brown
very tip colour
  brown
or    brownish
older parts colour
  black
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  not transparent
mantle laticifers visibility
  absent
mantle dots presence-colour
  brownish
or    concolorous to mantle
mantle carbonizing presence
  absent
mantle surface {in general} habit
  shiny
mantle surface {in detail} kind
  densely grainy or warty
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  concentrated proximally
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  cystidia
emanating elements cystidia location
  on outer mantle layer
blue granules presence
  present
blue granules location
  outer mantle layer
Anatomy of laticifers
presence
  absent
Anatomical features of the mantle
matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
organisation {if pseudoparenchymatous} cell shape
  angular
mantle type
  angular cells, bearing mounds of roundish cells (type K)
mound cells diameter
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mound cells granular contents
  absent
septa clamps presence
  absent
cell pigment location-colour
  membranaceously brownish
cell diameter
5 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
16 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
cell length
5 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
cell density
4     Lower value of unspecified range (could be µ-s.d., but not known)
8     Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2 µm    Maximum value
cell wall with globular thickenings
  absent
cell wall surface habit
  smooth
drops of exuded pigment presence
  absent
blue granules location
  within cells
Anatomy of the middle mantle layer
organisation
  pseudoparenchymatous
hyphae arrangement
  plectenchymatous, without pattern
cell pigment location-colour
  membranaceously brownish
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous
hyphae arrangement
  star-like
septa clamps presence
  present
cell pigment location-colour
  absent
or    membranaceously brownish
cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
8 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
23 µm    Upper value of unspecified range (could be µ+s.d., but not known)
38 µm    Maximum value
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
6     Lower value of unspecified range (could be µ-s.d., but not known)
9     Upper value of unspecified range (could be µ+s.d., but not known)
10     Maximum value
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
30 µm    Minimum value
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
80 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
mantle different layers presence
  discernable
outer mantle layer organisation
  pseudoparenchymatous
outer mantle layer hyphae tangentially length
4 µm    Minimum value
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
outer mantle layer hyphae radially diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
14 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
inner mantle layer hyphae radially diameter
1 µm    Minimum value
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
3     Maximum value
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
tangentially length
20 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
110 µm    Maximum value
radially diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
mean tangenial length TCt
53.1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
70.6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean shape-ratio TCq
7.2     Lower value of unspecified range (could be µ-s.d., but not known)
7.5     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented obliquely
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
45 µm    Lower value of unspecified range (could be µ-s.d., but not known)
120 µm    Upper value of unspecified range (could be µ+s.d., but not known)
130 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
7 µm    Minimum value
14 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
36 µm    Maximum value
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
75.6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
78.8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
3.7     Lower value of unspecified range (could be µ-s.d., but not known)
4.4     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  protruding towards endodermis
anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
or    two
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
structure {in plan view}
  of palmetti type
lobes width
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
Anatomy of the mantle in cross-section
mantle different layers presence
  discernible
outer mantle layer organisation
  pseudoparenchymatous
outer mantle layer hyphae tangentially length
3 µm    Minimum value
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
45 µm    Maximum value
outer mantle layer hyphae radially diameter
2 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
inner mantle layer organisation
  plectenchymatous
inner mantle layer hyphae tangentially length
1.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
15 µm    Maximum value
inner mantle layer hyphae radially diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value

Anatomy of the tannin cells in cross-section
presence
  present
rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
3     Maximum value
shape
  tangentially-oval to tangentially-elliptic
tangentially length
20 µm    Minimum value
23 µm    Lower value of unspecified range (could be µ-s.d., but not known)
36 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
radially diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
20 µm    Maximum value
mean tangential length TCt
30.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
32.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean shape-ratio TCq
3.1     Lower value of unspecified range (could be µ-s.d., but not known)
3.4     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in cross-section
anatomy mantle cross-section cortical (epidermal) cells shape
  tangentially-oval to tangentially-elliptic
anatomy mantle cross-section cortical (epidermal) cells tangentially length
12 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
65 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells radially diameter
7 µm    Minimum value
14 µm    Lower value of unspecified range (could be µ-s.d., but not known)
25 µm    Upper value of unspecified range (could be µ+s.d., but not known)
36 µm    Maximum value
anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
23.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
33.2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.2     Lower value of unspecified range (could be µ-s.d., but not known)
1.8     Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in cross-section
presence
  present
kind
  protruding towards endodermis
anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
anatomy mantle cross-section hyphal cells around tannin cells thickness
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
anatomy mantle cross-section hyphal rows around tannin cells number
  one
or    two
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
Anatomy of the emanating elements in general
intrahyphal hyphae presence
  present
Anatomy of cystidia

Anatomy of cystidia (type 1)
type
  awl-shaped, bristle-like (type A)
or    bottle-shaped with a straight neck (type B)
septa presence
  absent
or    present
septa kind
  absent, with basal clamp only
or    simple
diameter {proximal}
4 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter {distal}
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
length
10 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
54 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
cell wall colour
  brownish
cell wall colour {relative to mantle cells}
  similar
cell wall thickness {relative to mantle cells}
  thicker
cell wall evenness
  even in thickness
surface habit
  smooth
contents presence
  absent
contents type
  absent

Anatomy of emanating hyphae
shape
  not striking
cell pigment location-colour
  absent
or    membranaceously brownish
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  present
kind
  acute
or    approximately 90°
distance {from septum}
  adjacent to septum
or    one or two hyphal diam. below the septum
or    in considerable distance from the septum
side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
clamps presence
  present
clamps outline {in dorsal view}
  oval
clamps width {relative to hypha in dorsal view}
  thinner than
or    as broad as
clamps outline {in lateral view}
  less than a semicircle
or    not constricted at contact point to subtending hyphal cell
clamps width {relative to hypha in lateral view}
  thinner than
clamps hole presence
  absent

Anatomy of secondary septa of emanating hyphae
presence
  present
abundance
  abundant
distribution
  evenly distributed

Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
anatomy emanating elements emanating hyphae cell shape {at distal end}
  simple
anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
7 µm    Maximum value
anatomy emanating elements emanating hyphae cell length
22 µm    Minimum value
35 µm    Lower value of unspecified range (could be µ-s.d., but not known)
130 µm    Upper value of unspecified range (could be µ+s.d., but not known)
160 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
anatomy emanating elements emanating hyphae cell wall surface structures shape
  hemispherical warts
anatomy emanating elements emanating hyphae cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
2.5 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  as thick as
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs

Anatomy of hyphae in rhizomorphs
Anatomy of haustoria
{of ectomycorrhiza former} presence
  present
{of ectomycorrhiza former} abundance
  occasionally present
shape
  as normal hyphae
Anatomy of nuclei
number {per cell}
1     Minimum value
2     Mean (= average)
3     Maximum value
shape
  round
diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
length
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
Ultrastructure
septal pores type
  as dolipores with perforated parenthesome

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  absent
mantle in section blue-filter, 450-490 nm presence
  absent

Chemical reactions

reaction with acid fuchsin presence
  absent
reaction with anilin presence
  absent
reaction with brillant-cresyl-blue presence
  present
reaction with cotton-blue-lactic-acid presence
  present
reaction with ethanol 70% presence
  absent
reaction with FeSO4 presence
  absent
reaction with formol 40% presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  present
reaction with lactic acid presence
  present
reaction with Melzer's reagent presence
  absent
reaction with phenole presence
  present
reaction with ruthenium-red presence
  absent
reaction with sulpho-vanillin presence
  present
chemical compounds type
  thelephoric acid

Ecology

geographic occurrence continent
  Europe

Tree

plant family
  Pinaceae
plant genus
  Picea [abies]
plant habitat kind
  forests, woods

Fungus

family
  Thelephoraceae ss. Stalpers. excl. Tylospora

Remarks

public notes
  Mycorrhizal ends at most slightly bent; in cross-section tannin cells also half-moon shaped; mantle in sulfo-vanillin with violet cell walls and in total brownish-green, in KOH walls greenish and in total olive, in lactic acid walls brownish-ochre, in brillant-cresyl-blue violet, in cotton-blue walls greenish-brown, in phenole walls olive-green.

– Item listing –