Suillus collinitus (Fr.) O. Kuntze + Pinus

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– Enlarged view –

Literature

• references
  Uhl M (1988) Identifizierung und Charakterisierung von Ektomykorrhizen an Pinus silvestris und von Ektomykorrhizen aus der Gattung Tricholoma. Diss Uni München.

Morphology

Morphology of the mycorrhizal system
• length
3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  dichotomous
or    coralloid
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent
• exploration type
  long distance
Morphology of the unramified ends
• shape
  straight
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• diameter
0.35 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown
or    red
or    lilac, light reddish blue
• very tip colour
  brown
or    brownish
• older parts colour
  dark brown
or    brown
or    red
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  not smooth
• mantle surface {in detail} kind
  loosely woolly
• emanating hyphae presence
  present
• emanating hyphae abundance
  abundant
Morphology of rhizomorphs
• diameter
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.08 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• cross-section shape
  round or roundish
• connection to mantle kind
  distinct
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  ring-like arrangement of hyphal bundles (type A)
and    occasional patches of roundish cells on the mantle (type F)
• septa thickness {relative to cell walls}
  thinner than walls
• septa clamps presence
  absent
• cell pigment location-colour
  membranaceously yellowish
and    plasmatically yellowish
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.5 µm    Mean (= average)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  present
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• cell pigment location-colour
  membranaceously yellowish
or    plasmatically yellowish
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall thickness
0.2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  ring-like
• septa clamps presence
  absent
• cell diameter
2 µm    Mean (= average)
3 µm    Maximum value
Anatomy of the mantle in longitudinal section
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  plectenchymatous
• middle mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangenial length TCt
37.3 µm    Mean (= average)
• mean shape-ratio TCq
6.5     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  round
or    radially-oval to -elliptic
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
28.3 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
1.2     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• structure {in plan view}
  of palmetti type
• lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• outer mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• middle mantle layer organisation
  plectenchymatous
• middle mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
• middle mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in cross-section
• presence
  present
• rows number
1     Mean (= average)
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
15 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mean tangential length TCt
12 µm    Mean (= average)
• mean shape-ratio TCq
1.7     Mean (= average)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  round
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
27.5 µm    Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1.7     Mean (= average)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  protruding towards endodermis
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  roundish
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
0 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
or    two
Anatomy of the emanating elements in general
• septal pores configuration
  dolipore-like structures
• anastomoses type
  open, with a short bridge or bridge almost lacking
• anastomoses anastomosal bridge thickness {relative to hyphae}
  as thick as
Anatomy of cystidia


Anatomy of emanating hyphae
• drops of exuded pigment presence
  present


Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps width {relative to hypha in dorsal view}
  as broad as
• clamps hole presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  present
• abundance
  abundant
• distribution
  evenly distributed

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  even
• anatomy emanating elements emanating hyphae cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
or    without lens-shaped appositions
or    without spindle-shaped appositions


Anatomy of rhizomorphs
• type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
• nodia presence
  present
• internal nodia presence
  absent
• cup-like structures on surface presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  absent
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {of foreign origin} presence
  absent
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
• shape
  round
• diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• distance {between each other}
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of siderophilous granulations
• presence
  absent

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  absent
• mantle in section blue-filter, 450-490 nm presence
  present
• mantle in section green-filter, 530-560 nm presence
  absent

Chemical reactions

• reaction with acid fuchsin presence
  absent
• reaction with anilin presence
  present
• reaction with brillant-cresyl-blue presence
  present
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with FeSO4 presence
  absent
• reaction with formol 40% presence
  absent
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  present
• reaction with Melzer's reagent presence
  absent
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with sulpho-vanillin presence
  absent

Ecology

• geographic occurrence continent
  Europe

Tree

• plant family
  Pinaceae
• plant genus
  Pinus
• plant subgenus-section
  two-needle-pines
• plant habitat kind
  forests, woods

Fungus

• family
  Boletaceae
• subfamily
  Boletaceae subf. Suilloideae
• fruitbodies growth habit
  epigeous
or    pileate-porioid

Remarks

• public notes
  Mycorrhizal ends red-brown, very tips red-brown, older parts red-brown to dark brown; autofluorescence of mantle in section with blue-filter slightly yellowish; mantle in KOH drops of pigment becoming reddish, in brillant-cresyl-blue after addition of water margins of preparations slightly violet, in cotton- blue-lactic-acid drops of pigment light-blue, in anilin greyish brown.

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