Russula vinosa Lindbl. + Picea

– Item listing –

Literature

references
  Beenken L (2001) Russula vinosaLindbl.+ Picea abies(L.) H. Karst. Descr Ectomyc 5: 193-198.
Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, Universität München. http://edoc.ub.uni-muenchen.de/archive/00003175/01/Beenken_Ludwig.pdf

Morphology

Morphology of the mycorrhizal system
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
12 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  monopodial-pinnate
tips {per 10 mm} number
  unknown
ramification orders
  unknown
abundance
  unknown
main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.6 mm    Maximum value
taste {after 2-3 min chewing}
  unknown
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent [very]
exploration type
  medium distance smooth
Morphology of the unramified ends
mantle hydrophobicity presence
  present [at patches]
shape
  straight
shape {of distal end}
  not inflated, cylindric
length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
5 mm    Maximum value
diameter
0.3 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.4 mm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 mm    Maximum value
colour
  brown [light red-brown]
very tip colour
  brownish
after injury colour
  unknown
older parts colour
  unknown
mantle cortical cells visibility
  not visible
mantle {distinct} surface visibility
  present
mantle transparency
  unknown
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  whitish
mantle carbonizing presence
  absent
mantle surface {in general} habit
  not smooth
mantle surface {in detail} kind
  loosely grainy or warty
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  concentrated proximally
Morphology of rhizomorphs
diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.8 mm    Upper value of unspecified range (could be µ+s.d., but not known)
cross-section shape
  cushion-shaped (semicircular)
colour
  yellow
or    orange
after injury colour
  unknown
ramification kind-frequency
  unknown
connection to mantle kind
  distinct
origin location
  proximal
connection to substrate
  almost consistently growing along roots
margin habit
  hairy [cottony]
dimorphism presence
  unknown
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    emanating hyphae
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  present
Anatomical features of the mantle
completeness
  complete
matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
mantle type
  epidermoid cells bearing a hyphal net (type Q)
hyphal net cells shape
  triangular with obtuse ends
and    with ring- or star-like structures
hyphal net cells diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
hyphal net cell walls habit
  distinct, rather thick [0,3 µm]
hyphal net clamps presence
  absent
clavate end cell cover presence
  absent
hyphae arrangement
  not specific
hyphae sticky matrix on hyphal surface presence
  absent
pores between cells presence
  unknown
pores between cells abundance
  unknown
septa thickness {relative to cell walls}
  unknown
septa clamps presence
  absent
cell pigment location-colour
  membranaceously yellowish
cell contents presence-kind
  droplets, that stain in sulpho-vanillin
cell diameter
6 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell density
  unknown
cell wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Mean (= average)
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall with globular thickenings
  absent
cell wall surface habit
  smooth
cell wall projections presence
  present
cell wall projections abundance
  unknown
cell wall projections shape
  variable
drops of exuded pigment presence
  absent
roundish plasmatically yellow cells presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  present
organisation
  pseudoparenchymatous
hyphae arrangement
  plectenchymatous, without pattern
cell shape
  epidermoid
cell pigment location-colour
  membranaceously yellowish
cell contents presence-kind
  droplets, that stain in sulpho-vanillin
cell wall thickness
0.3 µm    Mean (= average)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall surface habit
  smooth
Anatomy of the inner mantle layer
organisation
  plectenchymatous [densely]
hyphae arrangement
  without pattern
hyphae vessel-like presence
  absent
septa thickness
  unknown
septa clamps presence
  absent
cell pigment location-colour
  membranaceously yellowish
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
or    pseudoparenchymatous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
50 µm    Maximum value
mantle thickness {at ectomycorrhizal tip}
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
calyptra cell remnants presence
  present
calyptra cell remnants type
  unknown
mantle different layers presence
  discernable
outer mantle layer organisation
  unknown
outer mantle layer hyphae tangentially length
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
27 µm    Upper value of unspecified range (could be µ+s.d., but not known)
outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer presence
  absent
inner mantle layer organisation
  unknown
inner mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
1     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis [flattened]
tangentially length
40 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
100 µm    Maximum value
radially diameter
10 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  rectangular
or    tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
60 µm    Lower value of unspecified range (could be µ-s.d., but not known)
100 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  in two rows, not reaching endodermis
anatomy mantle longitudinal section hyphal cells around tannin cells shape
  roundish [rectangular]
anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  roundish [rectangular]
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type [irregularly lobed]
lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae presence
  present
intrahyphal hyphae clamps presence
  absent
septal pores configuration
  unknown
backwards-oriented ramifications presence
  unknown
backwards-oriented clamps presence
  absent
anastomoses type
  open, with a short bridge or bridge almost lacking
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  straight
cell pigment location-colour
  absent
or    membranaceously yellowish
cell contents presence-kind
  absent
drops of exuded pigment presence
  absent


Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  unknown
anatomy emanating elements emanating hyphae cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5 µm    Maximum value
anatomy emanating elements emanating hyphae cell length
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
90 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.7 µm    Maximum value


Anatomy of rhizomorphs
type
  highly differentiated; thick hyphae forming mostly a core, septa often partially or completely dissolved
(type F)
nodia presence
  absent
internal nodia presence
  absent
gelatinous matrix presence
  present
gelatinized hyphae presence
  absent
hyphae distinctly different diameter
  absent
cup-like structures on surface presence
  absent
conical young side-branches presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
contents presence-kind
  absent
ampullate, trumpet-like inflated presence
  absent
roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs clamps presence
  absent
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
anatomy emanating elements rhizomorphs hyphae roundish plasmatically yellow cells presence
  absent
anatomy emanating elements rhizomorphs ladder-like hyphae presence
  present
anatomy emanating elements rhizomorphs ladder-like hyphae diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements rhizomorphs ladder-like hyphae wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{of foreign origin} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present [yellow-green throughout]
mantle in section blue-filter, 450-490 nm presence
  present [yellow throughout]
mantle in section green-filter, 530-560 nm presence
  present [red throughout]

Chemical reactions

reaction with acid fuchsin presence
  present [walls slightly pink]
reaction with cotton-blue-lactic-acid presence
  present [walls blue]
reaction with ethanol 70% presence
  present [droplets dissolving]
reaction with FeSO4 presence
  absent
reaction with guaiac presence
  absent
reaction with KOH 10% presence
  absent [droplets not dissolving]
reaction with lactic acid presence
  present [droplets not dissolving]
reaction with Melzer's reagent presence
  absent
reaction with sulpho-vanillin presence
  present [walls pink, contents of mantle cells with droplets becoming grey to black]

Ecology

substrate
  in organic layer
geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown

Tree

plant family
  Pinaceae
plant genus
  Picea
plant habitat kind
  forests, woods

Fungus

family
  Russulaceae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme Alu bands number
3     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 1. band from below number
390     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 2. band from below number
245     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme Alu bp of 3. band from below number
90     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
2     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
400     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
320     Mean (= average)

Remarks

public notes
  EcoRI: 410 bp, 300 bp; TaqI: 410 bp, 265 bp, 60 bp

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