Boletopsis leucomelaena (Pers. : Fr.) Fayod + Picea

– Item listing –

– Enlarged view –

Literature

• references
  Agerer R (1992) Studies on ectomycorrhizae XLIV. Ectomycorrhizae of Boletopsis leucomelaena (Thelephoraceae, Basidiomycetes) and their relationship to an unidentified ectomycorrhiza. Nova Hedwigia 55: 501-518.
Agerer R (1993) Boletopsis leucomelaena. In Agerer R (ed) Colour Atlas of Ectomycorrhizae, plate 75, Einhorn-Verlag, Schwäbisch Gmünd.

Morphology

Morphology of the mycorrhizal system
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
18 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• ramification presence-type
  monopodial-pinnate
• ramification orders
0     Lower value of unspecified range (could be µ-s.d., but not known)
2     Upper value of unspecified range (could be µ+s.d., but not known)
• abundance
  nest-like, hyphal mats
• main axis diameter
0.78 mm    Lower value of unspecified range (could be µ-s.d., but not known)
1.3 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• rhizomorphs as stout, short, conical structures presence-abundance
  absent
• rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
• rhizomorphs presence
  present
• rhizomorphs frequency
  infrequent
• exploration type
  medium distance mat
Morphology of the unramified ends
• shape
  bent
or    tortuous
• shape {of distal end}
  not inflated, cylindric
• length
0 mm    Lower value of unspecified range (could be µ-s.d., but not known)
5.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
6.5 mm    Maximum value
• diameter
0.27 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.39 mm    Upper value of unspecified range (could be µ+s.d., but not known)
• colour
  brown
or    yellow
or    white
• very tip colour
  brownish
or    violet, dark reddish blue
or    whitish
• older parts colour
  black
or    grey
• mantle cortical cells visibility
  not visible
• mantle {distinct} surface visibility
  present
• mantle transparency
  not transparent
• mantle laticifers visibility
  absent
• mantle dots presence-colour
  absent
• mantle carbonizing presence
  absent
• mantle surface {in general} habit
  silvery
or    smooth
• mantle surface {in detail} kind
  densely stringy
or    loosely stringy
Morphology of rhizomorphs
• cross-section shape
  round or roundish
or    flat
or    flat fans
• colour
  yellowish
or    white
• ramification kind-frequency
  repeatedly into smaller filaments
• origin location
  proximal
• margin habit
  hairy
• dimorphism presence
  absent
Morphology of sclerotia
• presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
• emanating elements presence-type
  rhizomorphs
• blue granules presence
  absent
Anatomy of laticifers
• presence
  absent
Anatomical features of the mantle
• matrix presence
  present
• matrix location
  outer mantle layer {apart from tip}
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
• organisation
  plectenchymatous
• mantle type
  hyphae rather irregularly arranged and no special pattern discernible (type B)
and    gelatinous matrix between the hyphae (type C)
• matrix kind
  gelatinous
• hyphae hyphal junctions angle {between}
  ca. 45° and less
• septa thickness {relative to cell walls}
  as thick as walls
• cell shape
  cylindric, constricted at septa
• cell pigment location-colour
  membranaceously brownish
and    membranaceously blackish
and    plasmatically brownish
• cell contents presence-kind
  absent
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• cell wall thickness
0.2 µm    Mean (= average)
• cell wall surface habit
  smooth
• drops of exuded pigment presence
  absent
Anatomy of the middle mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  plectenchymatous, without pattern
• cell pigment location-colour
  plasmatically brownish
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
6 µm    Maximum value
• cell contents presence-kind
  absent
• cell wall surface habit
  smooth
Anatomy of the inner mantle layer
• organisation
  plectenchymatous
• hyphae arrangement
  without pattern
or    ring-like
• septa clamps presence
  present
• cell diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
14 µm    Maximum value
• cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  plectenchymatous
• anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
2.5 µm    Minimum value
4 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
Anatomy of the mantle in longitudinal section
• mantle thickness {apart from tip}
10 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
70 µm    Maximum value
• mantle thickness {at ectomycorrhizal tip}
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• mantle different layers presence
  discernable
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
• middle mantle layer hyphae tangentially length
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
• presence
  present
• rows number
1     Minimum value
2     Mean (= average)
3     Maximum value
• shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis
• tangentially length
30 µm    Minimum value
55 µm    Lower value of unspecified range (could be µ-s.d., but not known)
150 µm    Upper value of unspecified range (could be µ+s.d., but not known)
180 µm    Maximum value
• radially diameter
10 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
28 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• mean tangenial length TCt
99.2 µm    Mean (= average)
• mean shape-ratio TCq
5.4     Mean (= average)

Anatomy of the cortical cells in longitudinal section
• anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
• anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
23 µm    Minimum value
30 µm    Lower value of unspecified range (could be µ-s.d., but not known)
130 µm    Upper value of unspecified range (could be µ+s.d., but not known)
167 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
15 µm    Minimum value
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
35 µm    Upper value of unspecified range (could be µ+s.d., but not known)
40 µm    Maximum value
• anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
72.6 µm    Mean (= average)
• anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
2.6     Mean (= average)

Anatomy of the Hartig net in longitudinal section
• presence
  present
• kind
  protruding towards endodermis
or    one or half a row of cortical cells adjoining endodermis free of Hartig net
• structure {in plan view}
  of palmetti type
• lobes width
1 µm    Minimum value
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of the mantle in cross-section
• mantle different layers presence
  discernible
• outer mantle layer organisation
  plectenchymatous
• outer mantle layer hyphae tangentially length
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• outer mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
• middle mantle layer hyphae tangentially length
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
• middle mantle layer hyphae radially diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
8 µm    Maximum value
• inner mantle layer organisation
  plectenchymatous
• inner mantle layer hyphae tangentially length
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• inner mantle layer hyphae radially diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
10 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in cross-section
• presence
  present
• shape
  tangentially-oval to tangentially-elliptic
• tangentially length
17 µm    Minimum value
23 µm    Lower value of unspecified range (could be µ-s.d., but not known)
46 µm    Upper value of unspecified range (could be µ+s.d., but not known)
60 µm    Maximum value
• radially diameter
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
27 µm    Maximum value
• mean tangential length TCt
35.6 µm    Mean (= average)
• mean shape-ratio TCq
3.5     Mean (= average)

Anatomy of the cortical cells in cross-section
• anatomy mantle cross-section cortical (epidermal) cells shape
  tangentially-oval to tangentially-elliptic
• anatomy mantle cross-section cortical (epidermal) cells tangentially length
12 µm    Minimum value
15 µm    Lower value of unspecified range (could be µ-s.d., but not known)
40 µm    Upper value of unspecified range (could be µ+s.d., but not known)
49 µm    Maximum value
• anatomy mantle cross-section cortical (epidermal) cells radially diameter
13 µm    Minimum value
17 µm    Lower value of unspecified range (could be µ-s.d., but not known)
38 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy mantle cross-section cortical (epidermal) cells mean tangential length CCt
23 µm    Mean (= average)
• anatomy mantle cross-section cortical (epidermal) cells mean shape-ratio CCq
1     Mean (= average)

Anatomy of the Hartig net in cross-section
• presence
  present
• kind
  one or half a row of cortical cells adjoining endodermis free of Hartig net
• anatomy mantle cross-section hyphal cells around tannin cells shape
  roundish
• anatomy mantle cross-section hyphal cells around tannin cells thickness
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8 µm    Upper value of unspecified range (could be µ+s.d., but not known)
10 µm    Maximum value
• anatomy mantle cross-section hyphal rows around tannin cells number
  two
or    three
or    several
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells shape
  cylindrical
or    beaded
• anatomy mantle cross-section hyphal cells around cortical (epidermal) cells thickness
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
• anatomy mantle cross-section hyphal rows around cortical (epidermal) cells number
  one
or    two
Anatomy of the emanating elements in general
• intrahyphal hyphae presence
  absent
• backwards-oriented ramifications presence
  present
• backwards-oriented clamps presence
  present
• anastomoses type
  open, with a short bridge or bridge almost lacking
or    closed by a clamp, with a short bridge or bridge almost lacking (contact-clamp)
• anastomoses cell wall thickness {relative to remaining cell walls}
  as thick as
• anastomoses anastomosal bridge thickness {relative to hyphae}
  thinner
or    as thick as
• anastomoses location
  not specified
Anatomy of cystidia


Anatomy of emanating hyphae
• shape
  not striking
• cell pigment location-colour
  membranaceously brownish
or    membranaceously yellowish
• drops of exuded pigment presence
  present

Anatomy of the ramification of emanating hyphae
• presence
  present
• kind
  acute
• distance {from septum}
  adjacent to septum
or    in considerable distance from the septum
• side-branches at septum number
  one side-branch at septum

Anatomy of the septa of emanating hyphae
• clamps presence
  present
• clamps width {relative to hypha in lateral view}
  thinner than
• clamps hole presence
  absent
• clamps blister-like structure {at basis} presence
  absent

Anatomy of secondary septa of emanating hyphae
• presence
  present
• abundance
  infrequent
• distribution
  not specified

Anatomy of the cells of emanating hyphae
• anatomy emanating elements emanating hyphae cell shape
  inflated, ampullate, trumpet-like at one side
• anatomy emanating elements emanating hyphae cell diameter
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall surface habit
  rough of warts
or    without lens-shaped appositions
or    without spindle-shaped appositions
• anatomy emanating elements emanating hyphae cell wall surface structures shape
  hemispherical warts
• anatomy emanating elements emanating hyphae cell wall thickness
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  even in thickness


Anatomy of rhizomorphs
• type
  slightly differentiated; central hyphae somewhat enlarged
(type C)
or    differentiated; some hyphae very thick, which appear randomly distributed, pores of septa somtimes enlarged
(type D)
• internal nodia presence
  absent
• gelatinous matrix presence
  present
• gelatinized hyphae presence
  absent
• conical young side-branches presence
  absent
• a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
• ampullate, trumpet-like inflated presence
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of peripheral hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
• anatomy emanating elements rhizomorphs clamps presence
  on central and peripheral hyphae
• anatomy emanating elements rhizomorphs hyphae ampullate, trumpet-like inflated presence
  absent
Anatomy of chlamydospores
• presence
  present
• type
  as oidia
• diameter
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of haustoria
• {of ectomycorrhiza former} presence
  absent
• {in foreign ectomycorrhizae} presence
  present
• {in foreign ectomycorrhizae} abundance
  occasionally present in a tip
• {of foreign origin} presence
  absent
Anatomy of nuclei
• number {per cell}
2     Mean (= average)
• shape
  round
• diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
• length
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2 µm    Upper value of unspecified range (could be µ+s.d., but not known)
Anatomy of siderophilous granulations
• presence
  present
• frequency
  in few cells
• diameter
0.5 µm    Mean (= average)

Autofluorescence

• whole mycorrhizae UV 254 nm colour-presence
  absent
• whole mycorrhizae UV 366 nm colour-presence
  absent
• mantle in section UV-filter 340-380 nm presence
  present
• mantle in section blue-filter, 450-490 nm presence
  present
• mantle in section green-filter, 530-560 nm presence
  present

Chemical reactions

• reaction with acid fuchsin presence
  absent
• reaction with anilin presence
  present
• reaction with brillant-cresyl-blue presence
  present
• reaction with cotton-blue-lactic-acid presence
  present
• reaction with ethanol 70% presence
  absent
• reaction with FeSO4 presence
  present
• reaction with guaiac presence
  absent
• reaction with KOH 10% presence
  present
• reaction with lactic acid presence
  absent
• reaction with Melzer's reagent presence
  amyloid
• amyloidity or dextrinoidity location
  patches of hyphal walls
• reaction with phenole presence
  absent
• reaction with phenole-aniline presence
  absent
• reaction with sulpho-vanillin presence
  present

Ecology

• substrate
  in organically enriched mineral horizon
or    in mineral soil
• geographic occurrence continent
  Europe
• knowledge about association with foreign fruitbodies presence
  known

Tree

• plant family
  Pinaceae
• plant genus
  Picea
• plant habitat kind
  forests, woods

Fungus

• family
  Bankeraceae ss. Stalpers
• fruitbodies growth habit
  epigeous
or    pileate-porioid

Remarks

• public notes
  Emanating hyphae at clamps up to 11 um thick; autofluorescence of mantle in section with UV-filter yellowish green and emanating hyphae near septa blue, with blue-filter mantle yellowish, with green-filter reddish; mantle in sulfo-vanillin brownish then hyphae dissolving, in KOH turning from yellowish to reddish brown, in FeSO4 greyish green and more distinctly so after addition of water, in brillant-cresyl-blue walls and cytoplasm slightly blue and after addition of water walls blue and cytoplasm violet, in cotton-blue walls and cytoplasm slightly blue, in anilin walls pink; distance of nuclei variable.

– Item listing –