Russula sardonia Fr. + Pinus

– Item listing –

Literature

references
  Mleczko P (2004) Russula sardonia Fr. + Pinus sylvestris L. Descr Ectomyc 7/8: 127-134. - Beenken L (2004) Die Gattung Russula. Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen. Dissertation, Universität München. http://edoc.ub.uni-muenchen.de/archive/00003175/01/Beenken_Ludwig.pdf

Morphology

Morphology of the mycorrhizal system
length
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
ramification presence-type
  dichotomous
or    coralloid
tips {per 10 mm} number
  unknown
ramification orders
  unknown
abundance
  unknown
main axis diameter
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
taste {after 2-3 min chewing}
  unknown
rhizomorphs as stout, short, conical structures presence-abundance
  absent
rhizomorphs as short mycorrhiza-like outgrowths with blunt tips presence
  absent
rhizomorphs presence
  present
rhizomorphs frequency
  infrequent
exploration type
  contact
Morphology of the unramified ends
mantle hydrophobicity presence
  absent
shape
  straight
shape {of distal end}
  not inflated, cylindric
length
0.5 mm    Lower value of unspecified range (could be µ-s.d., but not known)
3.5 mm    Upper value of unspecified range (could be µ+s.d., but not known)
diameter
0.3 mm    Minimum value
0.4 mm    Lower value of unspecified range (could be µ-s.d., but not known)
0.6 mm    Upper value of unspecified range (could be µ+s.d., but not known)
colour
  yellowish
or    reddish [rosy brown]
very tip colour
  yellowish
or    reddish [light rosy brown]
older parts colour
  brownish
or    yellow [dark]
or    orange [slightly]
or    reddish
mantle cortical cells visibility
  unknown
mantle {distinct} surface visibility
  present
mantle transparency
  unknown
mantle laticifers visibility
  absent
mantle secreted latex {after scratching mantle} presence
  absent
mantle dots presence-colour
  whitish
mantle carbonizing presence
  absent
mantle surface {in general} habit
  not smooth
mantle surface {in detail} kind
  densely grainy or warty
or    loosely grainy or warty
emanating hyphae presence
  present
emanating hyphae abundance
  infrequent
emanating hyphae distribution
  unknown
Morphology of rhizomorphs
diameter
  unknown
cross-section shape
  unknown
colour
  yellowish
ramification kind-frequency
  unknown
connection to mantle kind
  unknown
origin location
  proximal
margin habit
  unknown
dimorphism presence
  unknown
Morphology of sclerotia
presence
  absent

Anatomy

Anatomical features of the entire mycorrhiza
emanating elements presence-type
  rhizomorphs
or    emanating hyphae
emanating elements cystidia location
  unknown
crystals presence
  absent
blue granules presence
  absent
Anatomy of laticifers
presence
  absent
anatomy entire mycorrhiza laticiferous solitary (gloeoplerous) cells presence
  present
Anatomical features of the mantle
completeness
  complete
matrix presence
  absent
Anatomy of the outer mantle layer apart from the ectormycorrhizal tip
organisation
  pseudoparenchymatous
organisation {if pseudoparenchymatous} cell shape
  epidermoid, puzzle-like, jig-saw-shaped
mantle type
  some cells containing droplets, staining in sulpho-vanillin; shape variable (type N)
and    epidermoid cells bearing a hyphal net (type Q)
hyphal net cells shape
  aculeate, acuminated horn-shaped
and    with ring- or star-like structures
hyphal net cells diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
8.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
hyphal net cell walls habit
  distinct, rather thick
hyphal net clamps presence
  absent
hyphae arrangement
  not specific
hyphae sticky matrix on hyphal surface presence
  absent
pores between cells presence
  unknown
pores between cells abundance
  unknown
septa thickness {relative to cell walls}
  unknown
septa clamps presence
  absent
cell pigment location-colour
  membranaceously yellowish
cell contents presence-kind
  droplets, that stain in sulpho-vanillin
cell diameter
2 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
7.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
12 µm    Maximum value
cell length
5 µm    Minimum value
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
17 µm    Upper value of unspecified range (could be µ+s.d., but not known)
30 µm    Maximum value
cell density
3     Lower value of unspecified range (could be µ-s.d., but not known)
5     Upper value of unspecified range (could be µ+s.d., but not known)
cell wall thickness
0.3 µm    Minimum value
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1.7 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell wall with globular thickenings
  absent
cell wall surface habit
  smooth
cell wall projections presence
  present
cell wall projections abundance
  infrequent
cell wall projections shape
  even in thickness
drops of exuded pigment presence
  absent
roundish plasmatically yellow cells presence
  absent
pigment granula on surface presence
  absent
Anatomy of the middle mantle layer
presence
  present
organisation
  pseudoparenchymatous
hyphae arrangement
  not applicable
or    plectenchymatous, without pattern
cell shape
  epidermoid
cell diameter
  not applicable
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
12 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell length
  not applicable
8 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell density
  not applicable
cell contents presence-kind
  droplets, that stain in sulpho-vanillin
cell wall thickness
0.3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
0.3 µm    Mean (= average)
cell wall surface habit
  smooth
crystals diameter
  not applicable
crystals length
  not applicable
cell blue granules location
  not applicable
Anatomy of the inner mantle layer
organisation
  plectenchymatous [densely]
hyphae arrangement
  without pattern
hyphae vessel-like presence
  absent
septa thickness
  unknown
septa clamps presence
  absent
cell pigment location-colour
  membranaceously yellowish
cell diameter
2 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
5.5 µm    Maximum value
cell length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45 µm    Upper value of unspecified range (could be µ+s.d., but not known)
cell contents presence-kind
  absent
Anatomy of the outer mantle layer of the ectormycorrhizal tip
anatomy mantle outer mantle layer {of ectomycorrhizal tip} organisation
  like other parts of mantle
anatomy mantle outer mantle layer {of ectomycorrhizal tip} matrix kind
  not gelatinous
anatomy mantle outer mantle layer {of ectomycorrhizal tip} hyphae diameter
1.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
4.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle outer mantle layer {of ectomycorrhizal tip} cell density
  unknown
Anatomy of the mantle in longitudinal section
mantle thickness {apart from tip}
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
46 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mantle thickness {at ectomycorrhizal tip}
18 µm    Lower value of unspecified range (could be µ-s.d., but not known)
30.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
calyptra cell remnants presence
  unknown
calyptra cell remnants type
  unknown
mantle different layers presence
  discernable
outer mantle layer organisation
  plectenchymatous
outer mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
18 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
outer mantle layer hyphae radially diameter
2.5 µm    Minimum value
3 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
middle mantle layer organisation
  pseudoparenchymatous
middle mantle layer hyphae tangentially length
7 µm    Lower value of unspecified range (could be µ-s.d., but not known)
20 µm    Upper value of unspecified range (could be µ+s.d., but not known)
25 µm    Maximum value
middle mantle layer hyphae radially diameter
4.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
9.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
inner mantle layer organisation
  unknown
inner mantle layer hyphae tangentially length
5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
23 µm    Upper value of unspecified range (could be µ+s.d., but not known)
55 µm    Maximum value
inner mantle layer hyphae radially diameter
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
5 µm    Upper value of unspecified range (could be µ+s.d., but not known)

Anatomy of the tannin cells in longitudinal section
presence
  present
rows number
  unknown
shape
  tangentially-oval, -elliptic or -cylindrical, and oriented in parallel to root axis [often triangular and flattened]
tangentially length
11.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
45.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
radially diameter
5.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
38.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
mean tangenial length TCt
30 µm    Mean (= average)
mean shape-ratio TCq
1.94     Mean (= average)

Anatomy of the cortical cells in longitudinal section
anatomy mantle longitudinal section cortical (epidermal) cells shape
  tangentially-oval to -elliptic or -cylindrical, and oriented in parallel to root axis
anatomy mantle longitudinal section cortical (epidermal) cells tangentially length
11.5 µm    Minimum value
14 µm    Lower value of unspecified range (could be µ-s.d., but not known)
59.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells radially diameter
10.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
52.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section cortical (epidermal) cells mean tangential length CCt (ECt)
31 µm    Mean (= average)
anatomy mantle longitudinal section cortical (epidermal) cells mean shape-ratio CCq (ECq)
1     Mean (= average)

Anatomy of the Hartig net in longitudinal section
presence
  present
kind
  protruding towards endodermis
anatomy mantle longitudinal section hyphal cells around tannin cells shape
  unknown
anatomy mantle longitudinal section hyphal cells around tannin cells thickness
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around tannin cells number
  one
anatomy mantle longitudinal section hyphal cells around cortical (epidermal) cells shape
  unknown
anatomy mantle longitudinal section hyphal cells around cortical cells (epidermal) thickness
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
6 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy mantle longitudinal section hyphal rows around cortical (epidermal) cells number
  one
structure {in plan view}
  of palmetti type
lobes width
1 µm    Lower value of unspecified range (could be µ-s.d., but not known)
2.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
lobes septa presence
  unknown
Anatomy of the mantle in cross-section



Anatomy of the emanating elements in general
intrahyphal hyphae presence
  unknown
intrahyphal hyphae clamps presence
  absent
septal pores configuration
  unknown
backwards-oriented ramifications presence
  unknown
backwards-oriented clamps presence
  absent
anastomoses type
  open, with a short bridge or bridge almost lacking
anastomoses surface habit
  unknown
anastomoses cell wall thickness {relative to remaining cell walls}
  unknown
anastomoses anastomosal bridge thickness {relative to hyphae}
  unknown
anastomoses location
  unknown
Anatomy of cystidia


Anatomy of emanating hyphae
shape
  wavy
or    tortuous
cell pigment location-colour
  membranaceously yellowish
pigment presence-distribution
  unknown
cell contents presence-kind
  absent
drops of exuded pigment presence
  absent

Anatomy of the ramification of emanating hyphae
presence
  absent
kind
  acute
or    Y-shaped
distance {from septum}
  unknown
side-branches at septum number
  one side-branch at septum
anatomy emanating elements emanating hyphae point of ramification thickness {relative to main part of branch}
  unknown

Anatomy of the septa of emanating hyphae
clamps presence
  absent


Anatomy of the cells of emanating hyphae
anatomy emanating elements emanating hyphae cell shape
  even
anatomy emanating elements emanating hyphae cell shape {at distal end}
  unknown
anatomy emanating elements emanating hyphae cell diameter
2 µm    Minimum value
2.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
3 µm    Upper value of unspecified range (could be µ+s.d., but not known)
4 µm    Maximum value
anatomy emanating elements emanating hyphae cell length
20 µm    Lower value of unspecified range (could be µ-s.d., but not known)
60 µm    Upper value of unspecified range (could be µ+s.d., but not known)
anatomy emanating elements emanating hyphae cell wall surface habit
  smooth
anatomy emanating elements emanating hyphae cell wall thickness
0.3 µm    Minimum value
0.5 µm    Upper value of unspecified range (could be µ+s.d., but not known)
0.5 µm    Lower value of unspecified range (could be µ-s.d., but not known)
1 µm    Maximum value
anatomy emanating elements emanating hyphae cell wall layers number
  unknown
anatomy emanating elements emanating hyphae cell wall thickness at tip {relative to remaining cell wall}
  unknown
anatomy emanating elements emanating hyphae cell wall {apart from tip} evenness
  unknown


Anatomy of rhizomorphs
type
  undifferentiated; hyphae rather loosely woven and of uniform diameter
(type A)
internal nodia presence
  absent
gelatinous matrix presence
  absent
gelatinized hyphae presence
  absent
hyphae distinctly different diameter
  absent
cup-like structures on surface presence
  absent
conical young side-branches presence
  absent
a "ball" of intertwined, ramified, thin hyphae presence
  absent

Anatomy of hyphae in rhizomorphs
contents presence-kind
  absent
Anatomy of vessel-like or thickened hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of central non-vessel-like hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of peripheral hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of solitary thick-walled hyphae in rhizomorphs
anatomy emanating elements rhizomorphs hyphae contents presence-kind
  absent
Anatomy of chlamydospores
presence
  absent
Anatomy of haustoria
{of ectomycorrhiza former} presence
  absent
{in foreign ectomycorrhizae} presence
  unknown
{in foreign ectomycorrhizae} abundance
  unknown
{in foreign ectomycorrhizae} formed in fungal genus
  unknown
{of foreign origin} presence
  absent

Autofluorescence

whole mycorrhizae UV 254 nm colour-presence
  absent
whole mycorrhizae UV 366 nm colour-presence
  absent
mantle in section UV-filter 340-380 nm presence
  present [mantle blue]
mantle in section blue-filter, 450-490 nm presence
  present [mantle yellowish]
mantle in section green-filter, 530-560 nm presence
  present [mantle red]

Chemical reactions

reaction with acetic acid presence
  unknown
reaction with acid fuchsin presence
  unknown
reaction with AgNO3 presence
  unknown
reaction with anilin presence
  unknown
reaction with brillant-cresyl-blue presence
  unknown
reaction with cotton-blue-lactic-acid presence
  present [cell walls blue]
reaction with ethanol 70% presence
  unknown
reaction with FEA presence
  unknown
reaction with FeSO4 presence
  absent
reaction with formol 40% presence
  absent
reaction with guaiac presence
  absent
reaction with HCl presence
  unknown
reaction with KJ presence
  unknown
reaction with KOH 10% presence
  absent [15%]
reaction with lactic acid presence
  present [latex coagulating and eventually dissolving]
reaction with Melzer's reagent presence
  absent
reaction with methylene blue presence
  unknown
reaction with phenole presence
  absent
reaction with phenole-aniline presence
  absent
reaction with ruthenium-red presence
  unknown
reaction with sudan III presence
  absent
reaction with sudan IV presence
  unknown
reaction with sulpho-vanillin presence
  present [latex of gloeoplerous cells staining violet-black]
reaction with sulphuric acid presence
  variable
reaction with toluidin blue presence
  unknown
reaction with trypan blue presence
  unknown
reaction with water presence
  unknown

Ecology

soil pH
  pH 3 - 4
geographic occurrence continent
  Europe
knowledge about association with foreign fruitbodies presence
  unknown ['9001' added to avoid duplicates!]#OCCURS TWICE IN SAME CHARACTER: PLEASE CORRECT DEFINITION!#

Tree

plant family
  Pinaceae
plant genus
  Pinus
plant subgenus-section
  two-needle-pines
plant habitat kind
  forests, woods

Fungus

family
  Russulaceae
subgenus-section
  Russula sect. Firmae
fruitbodies growth habit
  epigeous
or    pileate-lamellate

DNA-Analysis

PCR- ITS1, -ITS4 restriction enzyme HinfI bands number
3     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 1. band from below number
380     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 2. band from below number
350     Mean (= average)
PCR- ITS1, -ITS4 restriction enzyme HinfI bp of 3. band from below number
120     Mean (= average)

Remarks

public notes
  EcoR I: 520 bp, 350 bp; Taq I: 300 bp, 260 bp, 130 bp, 75 bp, 65 bp, 55 bp

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